---

作 者 ：杜传印;刘洪祥;田纪春

期 刊 ：作物学报 2006年 32卷 10期 页码：1592-1596

关键词：烟草；种质资源；亲缘关系；AFLP；聚类分析；

Keywords:Tobacco, Germplasm resources, Phylogenesis, AFLP(Amplified Fragment Length Polymorphism), Cluster analysis,

摘 要 ：利用AFLP技术,对48份不同类型和地域来源的烟草种质的亲缘关系进行了分析。选用4对AFLP引物共检测到323条扩增带,其中174条具有多态性,平均多态检出率为55.72%。对AFLP扩增结果采用UPGMA法进行聚类分析,可以将48份烟草种质分为2大类群,即黄花烟草群和普通烟草群,后者又可进一步分为4组;48份材料的欧氏距离为1.41~10.78。初步研究表明,AFLP标记技术能较好地从分子水平揭示烟草种质资源的遗传背景和亲缘关系。

Abstract:Phylogenesis of 48 tobacco germplasms from different countries or regions was analyzed with amplified fragment length polymorphism(AFLP).Four pairs of AFLP informative primers generated 323 scorable bands in total,in which 174 bands were polymorphic and the average percentage of polymorphic bands was 55.72.Cluster analysis based on Euclidean distance by unweighted pair-group method using arithmetic average(UPGMA) clearly separated the 48 germplasms into two groups,namely N.tabacum L.group and N.rustica L.gr...

---