利用2对STS引物、1对SSR引物和1对基因特异引物分析了1 739份中国小麦地方品种Waxy基因的变异情况。检测出Wx-A1基因缺失突变材料3份，Wx-B1基因缺失突变材料25份，Wx-D1基因缺失突变材料3份。利用SDS-PAGE方法对以上Waxy基因突变材料在蛋白质水平上进行鉴定，验证了Waxy分子标记在种质资源研究中的有效性。向育种家推荐一批具有不同Waxy基因缺失的地方种质，并提供了全部品种的缺失类型。
A total of 1 739 Chinese wheat landraces were screened for the Waxy gene mutations with two STS, one SSR and one gene specific marker published in previous researches. Among of the landraces, 3 were null Wx-A1 mutants, 25 were null Wx-B1 mutants, and 3 were null Wx-D1 mutants. Different null types of Waxy genes were also certified by SDS-PAGE of waxy proteins, containing that these markers were effective to verify mutant and normal alleles at waxy loci. These markers can also be used in marker-assisted selection in breeding program. Furthermore, based on our findings, we recommend some accessions with different Waxy mutations to breeders. Genotypes of Waxy genes of these materials were also released in this paper.
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