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Easy Restoration of Fertility and Stability of Fertility Restoration for Male Sterile Line with Ae. kotschyi Cytoplasm in Wheat [Triticum aestivum (L.) Thell.]

小麦K型不育系的易恢性及育性恢复的稳定性研究


利用2个K型不育系与不同K型恢复系组配测交组合,研究了不同不育系的易恢性及其遗传机理,恢复系在年份间恢复度的稳定性以及播期、播量对育性恢复度的影响。结果表明, 2个K型不育系易恢性有明显差异,其易恢性受2对主基因和微效多基因共同控制,不育系易恢性与恢复系恢复力差异的机理是相同的。大多数K型杂交组合的恢复度在不同年份间有较大差异,但恢复度高的组合稳定性较好,异常年份鉴定恢复系的恢复力是最有效的。播期对恢复度的影响较大,播量影响较小。抽穗至开花期间的异常高温是恢复度不稳定的主要外部原因。

Male sterility of Ae. kotschyi cytoplasm is considered better than that of T. tomopheevi, but there are some problems restricting its application in field production, such as inductive haploid and fertility restoration. K-type hybrids with two sterile lines (A) and certain restoring lines (R) were used to study the easy restoration of fertility (ERF) in two A-lines, stability of fertility restoration in different years, and effects of sowing time and rate on restoring degree. The results showed that there was obvious difference in ERF of A-lines and that the sterile line Yumai 3 was easier to be restored than S43 (Fig.1 and Fig.2). Genetic analysis of three-way cross combination indicated that ERF were controlled by two main genes and some other quantitative genes (Fig.3 and Table 1). The mechanism of ERF for A-lines and of restoring ability for R-lines were the same. The restoring degree of most K-type hybrids differed greatly in different years, but the hybrids with higher restoring degree had good fertility restoring stability(Fig.4 and Fig.5), so it was the most effective to identify restoring ability of R-lines under abnormal climates. Sowing time had a great effect and sowing norm had a small effect on restoring degree of K-type hybrid (Table 2). Abnormal high temperature during the period from ear emergence to anthesis were the main environmental reason affecting stability of restoring degree.


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