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作 者 ：刘德华;周带娣;黎星辉;赵思东;陈庆余

期 刊 ：作物学报 1999年 25卷 03期 页码：291-295

关键词：茶树；外植体；胚性愈伤组织；体细胞胚；不定芽；

Keywords:Tea plant, Explant, Embryogenic callus, Somatic embryo, Adventitious bud,

摘 要 ：根据茶树子叶柄、叶柄断面离腋芽的距离，将外植体依次分为C1, C2, C3和L1, L2, L3。通过上述外植体和不同培养基的研究结果表明:子叶柄外植体在MT1和MT3培养基上培养，其不定芽及总分化率是C1>C2>C3;体细胞胚的分化率是C3>C2>C1。叶柄外植体只有L1在MT3培养基上培养分化了芽，MT1培养基的胚性愈伤组织诱导率比MT,培养基高，

Abstract:The explants of tea plant were classified into 6 types i.e. C1、C2、C3 and L1、L2,L3，according to the distances from cut of cotyledon petiole and leafstalk to axillary bud. The effect of the explant types on differentiation rates of somatic embryo and adventitious bud was studied.Adventitious bud

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