摘 要 ：天麻抗真菌蛋白(Gastrodia Antifungal Protein，GAFP)能强烈抑制腐生真菌菌丝的生长，在天麻限制和防止蜜环菌［Armillariella mellea (Vahl. ex Fr.)Karst.］侵染球茎的防卫机制中起重要作用。本文报告GAFP抗菌机理研究的部分内容——GAFP对木霉菌丝的作用位点。用荧光试剂异硫氰酸荧光素(Fluorescein isothiocyanate，FITC)标记GAFP，试验表明，标记后的GAFP与未标记的GAFP对木霉菌丝生长均有抑制作用。在荧光显微镜下观察GAFP在木霉菌丝上的作用位点，发现被“标记GAFP”作用后的菌丝边缘有荧光，并主要集中在木霉菌丝的顶端和菌丝横隔处，表明GAFP对木霉的作用位点在菌丝的细胞壁上。
Abstract:Pretreatment of maize (Zea mays L.,Qing No.3 and Da Huang) seeds with CaCl2 solution could increase survival rate of maize seedlings under chilling,heat,drought and salt stress.In contrast to this,pretreatment with the Ca2+chelator EGTA decreased survival rate of the seedlings under these stresses.The results demonstrated that the exogenous Ca2+ can improve maize seedlings multiresistance mentioned above.In addition,Ca2+ treatment of the seeds enabled the seedlings to keep relatively higher activity of glutathione reductase (GR) than that of Ca2+deficient treatment (control).EGTA treatment of the seeds led to more less of GR activity in the seedlings under these stress.It is obvious that GR is involved in the Ca2+enhanced the mutli resistance or the stress tolerances of the maize seedlings.