---

作 者 ：唐巍;欧阳藩;郭仲琛

期 刊 ：云南植物研究 1997年 19卷 03期 页码：1-3

关键词：火炬松；直接器官发生；植株再生；

Keywords:Pinus taeda,

摘 要 ：基因型 Hb，Ma和Mc的火炬松成熟合子胚在附加1.0 mg／LNAA，4.0 mg／L BA，500mg／LLH和 500 mg／L谷氨酰胺的TE培养基上培养12周后，在子叶和胚轴部位形成不定芽原基。然后将合子胚转移到附加0.5 mg／L NAA，0.5 mg／L IBA，2 mg／L BA，500 mg／LLH和 500mg／L谷氨酰胺的TE不定芽分化培养基上，6周后分化产生大量不定芽。3种基因型中，Hb的直接不定芽分化频率最高，达58.2％。直接不定芽经伸长培养6周后，转移到附加0.1 mg ／L IBA，1.0 mg／LBA和0.5 mg／LGA3的TE生根培养基上，6周后直接不定芽的最高生根频率达46％。

Abstract:Hb, Ma and Mc genotype mature zygotic embryos of loblolly pine were cultured on TE medium containing 1.0 mg / L NAA, 4.0 mg / L BA, 500 mg / L LH and 500 mg / L glutamine, and primary adventitious buds were formed on cotyledons and hypocotyl in 12 weeks. Zygotic embryos with primary adventitious buds were transferred to TE differentiation medium with 0.5 mg / L NAA, 0.5 mg / L IBA, 2 mg / L BA, 500 mg / L LH and 500 mg / L glutamine. After 6 weeks a lot of adventitious buds appeared. Among 3 genotypes, differentiation frequency of Hb genotype adventitious bud was 58.2% the highest. Direct adventitious buds which were cultured or elongation medium for 6 weeks were transferred to TE rooting medium supplemented with 0.1 mg / L IBA, 1.0 mg / L BA, 0.5 mg / L GA3, and the highest rooting frequency of adventitious bud was 46% in 6 weeks.

---