摘 要 ：根据美洲商陆（Phytolacca americana）种子中一种抗真菌蛋白 PAFP（Hu et al，1991）的N端部分氨基酸顺序，设计、合成一条5’端寡核苷酸引物，通过3’－RACE技术从种于总RNA扩增出一约350 hp的cDNA片段，成功地克隆到pGEM-T载体系统中。序列分析表明该cDNA含有114 hP的编码区，由此推导的38个氨基酸序列有36个与PAFP）的序列相同，仅在第35、36位氨基酸分别由Cys、Lys替代了后者的Gln和Ile。114hp的编码序列也被克隆。该cDNA可能编码一种新的38个氨基酸的抗真菌蛋白。
Abstract:Basing on the ammo acid sequences of 7 kD antifungal protein from Phytolacca americana ( Hu Zhong et al, 1991 ), We designed a pair of degenerated primers. By using 3‘ - RACE technique, we have d a cDNA fragment about 350 bp from the total RNA of the seeds of Phytolacca americana . PCR product was cloned into pGEM - T vectoy system directly, and the cDNA sequences was then determinated. The re-sults indicated that the cDNA fragment contains an encoding region about 114 bp, and that the deduced amino acid sequences is the same as the 36 amino acids of the PAFP protein, except the two amino acids of the No. 35 and No. 36, at which Gln and Ile is replaced by Cys and Lys respectively. In addition, the encoding region was cloned. The cDNA may encodea a new antifungal protein of 38 amino acids.