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作 者 ：江晓玲 俞守义 贺竹梅 彭志强 祁瑜

期 刊 ：云南植物研究 2004年 26卷 01期 页码：1-3

关键词：番茄；子叶外植体；转化系统；

Keywords:Allium mairei；Karyotype；Polymorphism；Diploid；Tetraploid；Bchromosome,

摘 要 ：目的：对番茄子叶外植体的转化进行优化，为进行转基因番茄的研究奠定基础。方法：通过正交设计实验，以子叶外植体的存活天数为观察指标，分别对农杆菌稀释浓度、浸染时间和转化方式等条件进行优化。结果：在子叶预培养2d、活化农杆菌稀释倍数约30倍、侵染时间为10min、共培养时间为2d的情况下，子叶存活时间最长，抗性芽产生频率达25％。结论：获得了高效的番茄子叶外植体转化系统，为进行基因的转化奠定了基础。

Abstract:The morphological and karyotipical variations of 8 local populations of Allium mairei Lévl.in Kunming area had been studied in this paper.The results showed that morphological characters of Amairei have abundant polymorphism in populations and obvious polytypism among populations，and the karyotype of Amairei is rather symmetry in SectRhiziridium.Diploid and Tetraploid populations were found in Amairei.The karyotypical formulae of diploid is 2n=2x=14m+2sm,and that of tetraploid is 2n=4x=28m+4sm.There is karyotypical polymorphism in Amairei because of the variations of polyploids，Bchromosomes and satellite chromosomes.

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