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作 者 ：卢善发 宋艳茹

期 刊 ：云南植物研究 1999年 21卷 04期 页码：1-3

关键词：植物激素；维管组织；分化；离体茎段嫁接；黄瓜；

Keywords:Plant hormones, Vascular tissue, differentiation, Explanted internode grafting, Cucumber,

摘 要 ：利用黄瓜(Cucumis sativus Linn.)试管苗离体茎段自体嫁接系统，研究IAA和ZT对砧木和接穗维管组织分化的影响，发现外源IAA和ZT是砧木和接穗间维管束桥分化的必要条件。培养基中外源激素的浓度和种类通过调控维管束桥形成时间和数目以及贯通砧木和接穗的管状分子数来调节嫁接体发育。接合部维管组织分化是生长素和细胞分裂素共同作用的结果。离体茎段自体嫁接系统是一个理想的研究植物维管组织分化的新系统。

Abstract:The induction of vascular tissues in graft unions by IAA zeatin (ZT) was studied by using explanted internode autografting of cucumber(Cucumis sativus) cultured in vitro. Exogenous hormones were the limiting and controlling factors for vascular bundle regeneration in the graft unions. Vascular development ,such as formation time and total amount of vascular bridges as well as tracheary elements(TE) between stocks and scions,depended on the concentrations and the kinds of exogenous homones. Explanted internode autografting is one of ideal systems for studying the vascular tissue differentiation.

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