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期 刊 ：植物遗传资源学报 2015年 16卷 1期

关键词：花生；高油酸；FAD2B；AS-PCR；琼脂糖凝胶电泳；近红光谱外分析；

Keywords:peanut, high oleate, FAD2B, agarose gel electrophoresis, AS-PCR, NIRS,

摘 要 ：本文利用等位基因特异性 PCR技术（Allele-specific PCR ，AS-PCR）对高油酸父本CTWE与4个低油酸母本组配的330个杂交后代进行分子鉴评，其中230个得到了539 bp的特异性条带。白沙1016 ?CTWE、海花1号 ?CTWE、冀0212-2 ?CTWE以及远杂9847 ?CTWE的杂交成功率分别为83.3 %、50.0 %、57.1 %和 50.0 %。本研究采用单粒近红外光谱分析法对F2：3家系进行检测，结果表明远杂9847 ?CTWE、白沙1016 ?CTWE、冀0212-2 ?CTWE以及海花1 ?CTWE的F2:3家系中，全部为高油酸类型的家系分别为9个、8个、2个和3个，推断F2群体中，基因型为FAD2B-m/FAD2B-m的个体分别占F2群体的9.47 %、4.17 %、3.39 %和3.37 %。四个杂交组合的高油酸性状的遗传在p=0.05水平上符合两对基因的遗传模式。本研究结果对于高油酸性状的分子鉴定、高油酸花生新品种的培育以及育种效率的提高具有一定的参考价值。

Abstract:Allele-specific PCR (AS-PCR) was adopted to molecular evaluation to 330 hybrids from 4 hybridization combinations, which only one male parent was high oleate peanut line CTWE and female parents were 4 normal oleate conventional varieties. The results of agarose gel electrophoresis showed only 230 hybrids had a specific 539 bp band. Hybridization success rates of Baisha 1016 ?CTWE, Haihua1 ?CTWE , Ji 0212-2 ?CTWE and Yuanza9847 ?CTWE were 83.3 %, 50.0 %, 57.1 % and 50.0 % respectively. The Near-Infrared Spectroscopy analysis (NIRS) for single kernal was adopted to measure the F2:3 family lines of Yuanza9847 ?CTWE ,Baisha 1016 ?CTWE, Ji 0212-2 ?CTWE and Haihua1 ?CTWE. High oleic family lines were 9,8,2 and 3 respectively, which deduced the ratio of FAD2B-m/FAD2B-m in F2 population was 9.47 %, 4.17 %, 3.39 % and 3.37 % respectively. The genetic pattern of the high oleic trait of 4 hybridization combinations all accorded with 2 pairs of genes at p=0.05 level . This study has certain reference value for the chromosomal identification for high oleic trait and also could increase the efficiency of high oleate peanut breeding.

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