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作 者 ：王钧;万石双;李英

期 刊 ：云南植物研究 1981年 3卷 04期 页码：1-3

关键词：冠瘿；诱导；培养；植株；再生；

Keywords:induction, culture, plant, crown, calls,

摘 要 ：用根癌土壤杆菌S－1、702和C－58从向日葵胚轴、烟草茎和茎髓，胡萝卜根及马铃薯块茎诱发并建立了冠瘿株系。观察了它们的生长，畸胎瘤发生。在诱发后数月内有些冠瘿株发生明显变化，向日葵冠瘿株在诱发七个月后外形和生长速度发生了变化。由S－1菌株诱发的马铃薯块茎畸胎瘤发生在诱发初期，但702菌株诱发的两株烟草无组织冠瘿株在诱发后155天左右才出现畸胎瘤，不同菌株诱发的两个烟草冠瘿株对植物激素的反应不同。获得了分化程度不同的一组畸瘤胎。用植物激素研究了冠瘿瘤的逆转。两个胡萝卜和两个烟草冠瘿株系分别得到再生植株。所有冠瘿株包括畸胎瘤产nopaline，由烟草畸胎瘤的再生植株也测出了nopaline。

Abstract:Crown gall strains caused by Agrobacterium tumefaciens S-1 702, C-58 have been established from sunflower hypocotyls, tobacco stems and stem pithes,carrot roots and potato tubers. The growth of galls and the appearance of teratomas were investgated. Some crown galls strains changed properties many months after induction. A sunflower crown gall strain changed its shape and growth speed seven months after induction. The teratoma of potato tuber aused by A. tumefaciens S- 1 appeared in the early stage of crown gall induction, but the teratomas arisen from two tobacco unorganized crown gall strains caused by A. tumefaciens 702 about 155 days after induction. Two tobacco crown gall strains caused by different A. tumefaciens strains have different response to phytohomone. Several teratoma strains with different differentiation have been obtained. The reversion of crown galls was investigated by means of induction with phytohormone. Plantlets have been induced from two carrot crown gall strains and two tobacco crown gall strains. All crowngall strains and teratomas produce nopaline. Nopaline was detected from the tobacco plantlet inducted from teratoma.

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