Abstract:Large amounts ot viable protoplast were isolated from ealli of D.ianthus, caryophyllus. L. It is necessary that bovine serum albumin and potas-sium dextran sulfate were involved in enzyme mixtures for obtaining survivmg and dividing protoplats. Comparative experiments were studied on different bas,c culture medium, such as B,, MS and KM8p. Sustained division of carnahon protoplast can be obtained only on B. medium supplemented with 2 mg/1 glycme 04 mg/1 folic acid, 0.05 mg/1 biotin, 100 mg/1 L-glutamic acid, 100 mg/l L-aspartie acid, 100 mg/1 casein hydrolysate. 1 mg/l 2,4-D, 0.1 mg/1 NAA,0.5 m,/l 6-BA.100 ml/1 coconut milk, 60 g/1 mannitol and 30 g/1 sucrose, pH 5.7. Calli can be seen with naked eye after one month in culturing. Differentiation of calli derived from protoplast is in progress, but it is difficult.