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期 刊 ：植物遗传资源学报 2014年 15卷 3期

关键词：绿豆；PEG-6000；芽期；抗旱性；相关性分析；隶属函数；

Keywords:Mungbean, PEG-6000, Bud Stage, Drought resistance, Correlation analysis, Subordinative function analysis,

摘 要 ：干旱是影响我国绿豆生产的主要因素之一，筛选抗旱性种质资源，培育抗旱品种，对我国绿豆产业发展具有重要意义。本研究首先以4份绿豆种质为材料，通过检测5个梯度渗透势PEG-6000溶液模拟旱胁迫下的发芽率动态分布，确定了绿豆芽期模拟旱胁迫的适宜PEG-6000溶液渗透势为-0.9MPa（21.8%）。进而以-0.9MPa的PEG-6000溶液对113份绿豆种质进行芽期抗旱鉴定，通过测定发芽势、发芽率、下胚轴长、胚根长、干重和鲜重等10项指标及相关性分析，认为相对发芽势、相对发芽率、相对下胚轴长、相对胚根长、相对总芽长、相对鲜重、相对干重、相对发芽指数、相对活力指数9项指标可以作为评价绿豆芽期抗旱性指标。利用隶属函数分析法，对113份种质的芽期抗旱性进行综合评价，筛选出1份高抗种质当地吉豆（C0000626）和16份抗性种质，为绿豆抗旱基因发掘及抗旱品种改良奠定基础。

Abstract:Drought stress is a major problem for mungbean production and the drought tolerance evaluation is a priority research for mungbean in whole world. The germination rate of four accessions of mungbean germplasm resources was tested with 5 PEG-6000 density. The results showed that 21.8% (-0.9MPa) of PEG-6000 is the suitable density for screening drought resources at bud stage for mungbean. The drought resistance of 113 accessions of mungbean germplasms resources were evaluated with -0.9MPa PEG-6000 solution. Correlation analysis results showed that relevant germination energy, relevant germination rate, relevant hypocotyl length, relevant radical length, relevant total length, relevant fresh biomass, relevant dry biomass, relevant germination index and relevant vigor index were closely related to the drought resistance. Based on the average membership function analysis, the highest drought resistant germplasm, Dangdijidou (C0000626) and 16 drought resistance germplasms were identified and recommended to be used in the drought tolerance improvement for mungbean breeding.

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