---

期 刊 ：植物遗传资源学报 2015年 16卷 5期

关键词：大豆；miR-171；基因家族；靶基因预测；

Keywords:Soybean, miR-171, Gene family, Target gene predicting,

摘 要 ：本文运用生物信息学方法在miRBase搜索大豆miR-171（gma-miR-171）基因家族的序列，分析gma-miR-171序列的进化特征并预测其靶基因。结果表明，在miRBase中共搜索到21条gma-miR-171基因家族序列。序列分析发现，gma-miR-171基因家族序列保守型较差，只有2个碱基完全保守。对gma-miR-171基因进行定位，21个成员分散在12条染色体上，其中Chr06上gma-miR-171基因最多，共4个。进化分析表明，位于同一条染色体上的gma-miR-171基因没有表现出较近的亲缘关系。靶基因预测获得14个gma-miR-171基因家族的靶基因，包括蛋白激酶、磷酸酶、输出蛋白、转录因子等，说明gma-miR-171基因家族在大豆中具有广泛的调控功能。

Abstract:The miR-171 gene family of soybean (gma-miR-171) was searched in miRbase using bioinformatics method in this study. Molecular evolution of gma-miR-171 gene family was analyzed and their target genes were predicted. The results showed that, 21 sequences of gma-miR-171 gene family were found in miRBase. Sequences analysis revealed that the gma-miR-171 gene family had low conservation. Only 2 bases were conserved completely. Gene location suggested that 21 gma-miR-171 genes were distributed in 12 chromosomes. Among them, Chr06 had the most gma-miR-171 genes distribution, and there were 4 gma-miR-171 genes locating in Chr06. Evolution analysis showed that the genes located in the same chromosome did not have nearer phylogenetic relationship than other gma-miR-171 genes. Gene predicting found 14 target genes of gma-miR-171 gene family, including proteinase, phosphatase, exportin, transcription factor. These findings suggest that gma-miR-171 gene family plays significant roles in soybean.

---