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期 刊 ：植物遗传资源学报 2014年 15卷 5期

关键词：普通菜豆；镰孢菌枯萎病；水杨酸；系统获得抗性；

Keywords:Common bean, Fusarium wilt, Salicylic acid, System acquired resistance,

摘 要 ：普通菜豆（Phaseolus vulgaris L.）是人类主要食用豆类之一，其营养价值高，栽培面积大。镰孢菌枯萎病是普通菜豆典型的土传病害，给普通菜豆生产带来严重损失。水杨酸（SA）被认为是诱导植物抗病反应的重要信号分子之一，参与植物的过敏反应（HR）和系统获得性抗性反应（SAR）。本研究通过不同植物激素处理普通菜豆BRB-130，结果表明，SA处理普通菜豆叶片使植株根中SA的含量升高，并显著提高植株对枯萎病原菌FOP-DM01菌株的抗性。SA诱导普通菜豆根组织中苯丙氨酸解氨酶（Phenylalanine ammonia lyase, PAL）、过氧化物酶（Peroxidases，POX）活性及过氧化氢（Hydrogen peroxide，H2O2）的含量显著升高，从而诱导普通菜豆产生HR和SAR。因此，SA做为普通菜豆抗病信号途径中重要的化学激活因子，能够显著提高普通菜豆对枯萎病原菌（Fusarium oxysporum f. sp. phaseoli）的抗病性，为发展环境友好型化学农药提供新的思路。

Abstract:Common bean is one of major food legumes with high nutrition and large production area. Fusarium wilt has been a seriously soil-born disease and caused a great loss to the production of common beans. Salicylic acid (SA) is considered to be one of the important signaling molecules involved in plant defence reactions and has been proved to be involved in plant hypersensitive reaction (HR) and systemic acquired resistance (SAR). In this study, we treated the leaves of common bean cultivars BRB-130 by different plant hormones. The results indicated the levels of endogenous SA in bean roots were increased when the leaves were treated by SA; the resistance to Fusarium oxysporum f. sp. phaseoli isolate FOP-DM01 was significantly improved; the enhancements of PAL and POX activity and H2O2 level were all detected significantly in roots. SA-treatment induced HR and SAR in common beans. Therefore, SA has the ability to improve resistance to Fusarium wilt of common beans as an important chemical activator in the resistant signaling pathways. This study provides a new idea for the development of environment-friendly fungicides.

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