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期 刊 ：植物遗传资源学报 2015年 16卷 4期

关键词：番茄；B3基因家族；生物信息学；

Keywords:Tomato, B3 superfamily, bioinformatics,

摘 要 ：B3超家族是一类含有B3功能域（与DNA结合的高度保守结构域）的转录因子，在植物生长发育过程中起关键作用。本文采用生物信息学的方法，利用Pfam中的B3保守结构域序列检索番茄（Solanum lycopersicum L.）蛋白序列，确定了97个B3超家族基因。对番茄B3超家族成员进行了系统进化树分析、染色体定位、结构域分析、组织表达和诱导表达分析等。番茄B3超家族分为LAV、ARF、RAV和REM 四个亚家族，每个亚家族中的数量分别为4、22、9和62个，且在进化树中形成明显不同的分支，每个亚家族都进行了系统进化和结构域分析；番茄12条染色体都含有B3超家族基因；部分番茄B3超家族基因具有不同的组织表达模式，在各个发育时期均有表达并且响应不同的外界信号。本研究将为B3基因超家族成员的生物学功能研究提供参考。

Abstract:The B3 superfamily plays vital roles in plant growth and development, especially in related signal transduction pathway as a kind of transcription factors. All members of the B3 superfamily share the B3 DNA-binding domain of approximately 110 amino acids. 97 members of the tomato B3 superfamily were all confirmed according to BLAST from the protein databases of tomato using the conserved B3 domain. Phylogenetic trees, chromosomal localization, function domain of all B3 members, tissue and induced expression patterns of partial B3 members were all analyzed. All the members were divided into four subfamilies, including LAV, ARF, RAV and REM, with 4, 22, 9 and 62 members, respectively. The branch of each subfamily was obviously distinguished from others in the phylogenetic tree, with analyzing the function domains of all members. All members of the B3 superfamily were assigned to each chromosome, and each subfamily distributed in different patterns and frequency. Partial members of B3 superfamily have different tissue and induced expression patterns, and responded to different signals.

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