Abstract:Calcium is a critical messenger in abiotic stress responses signal transduction pathways in plants. Calcineurin B-like proteins (CBLs) represent a family of plant calcium sensor protein that function in calcium signaling by interacting with their interacting protein to transduce signal, making plant response to abiotic stress. Our laboratory has identifies a putative soybean CBL gene that functions in abiotic stress tolerance tolerance in the transgenic Arabidopsis plants. In order to further explore the GmCBL1-mediated stress resistance mechanism, the mixture of bait vectors pGBKT7-GmCBL1 were constructed, pGADT7 and soybean cDNA library was introduced into yeast competent cells AH109 to screen the interaction proteins from soybean cDNA library by yeast-two hybrid system. A total of 106 candidate positive clones were sequenced, analyzed through BLAST. According to their possible physiological function , those candidate proteins were classified into one of four categories: energy metabolism proteins, modified protein, defense protein, calcium signaling transduction proteins. Function prediction of the candidate proteins suggested that GmCBL1 was possibly involved in several stress signal transduction pathways.This work provides a basis for a defined further functional dissection of soybean CBL mediated signaling system.