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期 刊 ：植物遗传资源学报 2014年 15卷 3期

关键词：抗PVY；烟草；SSR；多样性分析；

Keywords:Tobacco, Resistance to PVY, SSR, Genetic diversity analysis,

摘 要 ：利用SSR标记对 78份抗PVY烟草种质进行遗传多样性分析，根据类型将78份材料分为烤烟、晒晾烟和黄花烟3个群体。结果表明：（1）从960对引物组合中筛选出45对扩增带型清晰、重复性和多态性好的引物用于 78 份烟草种质资源遗传多样性研究，共检测到108个位点，其中96个位点具有多态性，多态性比例为 88.89%。遗传多样性指数（Nei‘s）为0.3766，Shannon‘s 指数为0.5821，种质间遗传相似系数变异范围在 0.0454-0.9973之间，说明我国抗PVY烟草种质资源存在着丰富的遗传变异。（2）3个群体内遗传差异由大到小分别为：晒晾烟＞黄花烟＞烤烟。3个群体间遗传距离比较，晒晾烟与烤烟的遗传相似度达到0.9840。（3）聚类分析可将 78 份种质材料划分为黄花烟草和普通烟草 2 大类群，烤烟、晒晾烟之间没有明确的界线。聚类分析计算结果表明，黄花烟与其它群体的遗传距离较远。

Abstract:Levels of genetic variation within 78 tobacco germplasm resistance to PVY, including three populations (Flue-cured 18, sun-cured 38, N.rustica L. 22), were investigated using simple sequence repeats (SSR). The results were as follows：(1)Totally 108 loci were produced with 96 polymorphic loci (88.89%) using 45 different primer combinations selected from 960 primers combinations. A high level of genetic differentiation among populations was detected based on Nei‘s genetic diversity analysis (0.3766), Shannon‘s information index analysis (0.5821) and the genetic similarity coefficient variation range（0.0454-0.9973）.(2) The genetic differentiation was different within the three populations: Sun-cured＞N. rustica L.＞Flue-cured. Compared the genetic distances among three groups, the similarity of mahogany and flue-cured tobacco genetic is 0.9840. (3) The cluster analysis indicated 78 tobacco germplasms were divided as N. rustica L. and N. tabacum L. There exists not a clear boundary line between Sun-cured and Flue-cured. The genetic relationship among populations was studied by Nei unbiased genetic coefficient and Nei unbiased genetic distance, clustering analysis. The genetic distance was far between N.rustica L. population and the other N.tabacum L. populations.

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