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期 刊 ：植物遗传资源学报 2014年 15卷 1期

关键词：基因组大小；樟树；杂合率；GC含量；

Keywords:genome size, Cinnamomum camphora, hybridity percentage, GC_depth,

摘 要 ：樟树是我国特有的珍贵用材和经济树种，它富含多糖多酚、萜类等次生代谢物质，是香精香料、油脂化工、医药等的重要原料树种。本研究采用高通量测序技术（Illumina HiseqTM 2000）首次测定了樟树基因组大小，并利用生物信息方法估计樟树杂合率、重复序列情况、GC含量等基因组信息，为全基因组测序策略的选择提供依据。主要结论如下：（1）樟树基因组大小粗略估计为760 Mb左右；（2）樟树基因组有较高的杂合和一定的重复，杂合率约为0.65%；（3）由于樟树杂合率较高，全基因组鸟枪法策略不适合该基因组测序分析，可尝试使用BAC-to-BAC策略或fosmid策略，有利于樟树基因组的序列拼接和组装。

Abstract:Cinnamomum camphora (L.) Presl is a kind of precious wood species and the main no-timber trees of special local product in our country and possess abundant the secondary metabolism，such as polysaccharides, polyphenols, oleicacid and terpenoid. C. camphora is a metallic element used in many important industries. In this study, the genome size of C. camphora was determined by new-generation sequencing technologies (Illumina HiseqTM 2000). Hybridity percentage, repeats and GC_depth were also estimated by bioinformatics. The main results were showed as following: (1) The average genome size of C. camphora was about 760 Mb; (2) Hybridity percentage of C. camphora was about 0.65%; (3) Whole-genome shotgun sequencing was not used to C. camphora genome sequencing, and the BAC-to-BAC or fosmid could be used to it.

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