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Effects of combined administration of Paeoniae Radix Alba on local pharmacokinetics of six aconite alkaloids by skin microdialysis in vivo

在体皮肤微透析法研究制川乌白芍配伍对6种酯型生物碱局部药动学的影响



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EfectsofcombinedadministrationofPaeoniaeRadixAlbaonlocal
pharmacokineticsofsixaconitealkaloidsbyskinmicrodialysisinvivo
LIXiaoli,WULu,WUWeigang,YANGHuasheng
(SchoolofPharmacy,JiangxiUniversityofTraditionalChineseMedicine,Nanchang330004,China)
[Abstract] TheAconitiRadixCoctagelandAconitiRadixCoctacombinedwithPaeoniaeRadixAlbagelwereadministeredtomice
PhysiologicalsalinewastakenasperfusateTheperfusionratewas2μL·min-1andthemicrodialysissampleswerecolectedevery
05hintervalsforeighttimesThesixaconitinealkaloidsconcentrationinperfusateweredeterminedbyHPLCMS/MSTheconcen
trationtimecurveswereploted,andthepharmacokineticparameterswerecalculatedandanalyzedbySPSSTheefectsofPaeoniae
RadixAlbaontransdermalpermeationroleofsixaconitinealkaloidsinherbcoupleofPaeoniaeRadixAlbaAconitiRadixCoctawere
investigatedAccordingtotheresults,TmaxofthethreemonoesteraconitumalkaloidsofAconitiRadixCoctacombinedwithPaeoniae
RadixAlbagroupswereshortened,meanwhile,CmaxandAUCofbenzoylaconineandbenzoylhypaconinewereincreased.However,
AUCofthethreediestertypealkaloidswerereduced,withTmaxofhypaconitineprolongedandCmaxloweredThestudysuggestedthat
thecombinedadministrationofAconitiRadixCoctaandPaeoniaeRadixAlbapromotedthetransdermalpermeationofmonoesteraconi
tumalkaloids,andinhibitedtheabsorptionofpartsofdiestertypealkaloidsThisstudyprovedthedecreasingtoxicityandincreasing
eficacyofthecombinationofAconitiRadixCoctaandPaeoniaeRadixAlbaonthetransdermalpermeation,andprovidedareference
forstudiesontheprescriptioncombinationregularityandrelevantpractices
[Keywords] combinedadministration;transdermalpermeation;microdialysis;AconitiRadixCocta;PaeoniaeRadixAlba;HPLC
MS/MS
doi:10.4268/cjcmm20160530
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Table2 Invitromicrodialysisrecoverybygainandloss(珋x±s,n=5)
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100 2138±142 2093±174
300 1985±045 1947±156
BA 10 2296±091 2237±054 2249±218 2262±026
100 2225±099 2292±299
300 2189±089 2246±189
BH 10 2602±225 2443±155 2535±202 2511±134
100 2434±130 2367±150
300 2292±090 2631±101
MT 10 3882±294 3747±117 3901±294 3875±081
100 3678±150 3940±241
300 3681±191 3784±245
AT 10 4467±284 418±248 4280±153 4081±178
100 4031±175 3937±215
300 4044±190 4027±340
HT 10 2457±150 2214±219 2273±310 2231±085
100 2156±070 2133±311
300 2030±110 2288±156
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AUC0t/mg·h·L-1 29304±13361 17329±5060
MRT0t/h 257±031 297±040
BA Tmax/h 14±0421) 21±055
Cmax/mg·L-1 6885±27932) 1870±547
AUC0t/mg·h·L-1 13643±45772) 6169±1930
MRT0t/h 260±023 318±047
BH Tmax/h 14±0421) 21±055
Cmax/mg·L-1 7681±35802) 2242±667
AUC0t/mg·h·L-1 14332±52192) 6616±1851
MRT0t/h 275±015 3067±048
MT Tmax/h 16±042 1±071
Cmax/mg·L-1 4234±1878 6798±2162
AUC0t/mg·h·L-1 8295±30712) 18056±6902
MRT0t/h 287±019 260±036
AT Tmax/h 11±065 1±071
Cmax/mg·L-1 7286±6450 7584±2197
AUC0t/mg·h·L-1 11594±83741) 21798±7850
MRT0t/h 243±045 271±032
HT Tmax/h 14±0421) 1±071
Cmax/mg·L-1 9009±40292) 14519±4214
AUC0t/mg·h·L-1 19137±67642) 39153±12501
MRT0t/h 282±021 274±054
  


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