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期 刊 ：植物遗传资源学报 2012年 13卷 2期

关键词：薏仁米；种质；遗传多样性；SSR；

Keywords:YiRen rice, Germplasm, Genetic diversity, SSR,

摘 要 ：利用SSR 标记研究了 22个薏仁米种质的遗传多样性，用11对扩增带型稳定的SSR引物从供试材料中检测出105个等位基因变异，每对引物检测等位基因4～20个，平均9.55个。 SSR 引物的 PIC介于0.3048～0.9238，平均多态性信息量为0.8255。利用 UPGMA 聚类分系法将供试自交系划分为 4类，该划分结果与根据地理来源、种质系谱的分类结果基本一致。SSR分子标记辅助的种质改良是薏仁米品种改良的重要途径。

Abstract:The genetic diversity of 22 YiRen rice germplasm were studied by simple sequence repeats (SSR)markers. 11 SSR primers giving stable amplified band pattern detected 105 alleles among the lines tested. The average number of alleles per SSR locus were 9.55 with a range from 4 to 20. The value of polymorphism information content (PIC) for each SSR locus varied from 0.3048 to 0.9238 with average of 0.8255. The 22 germplasm were divided into 4 groups by UPGMA method based on SSR fingerprinting. The clustered results were similar to that based on geograph-ical resource and germplasmic genealogy. SSR marker could be an accurate and reliable method to study diversity of YiRen rice Germplasm. SSR marker assisted genetic improvement of YiRen rice germplasm would be an important way to breed new YiRen rice cultivars.

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