作 者 :姜超,王文竹,廖小军,曾德泉,林挺,徐士兰,曾锦章,陈海峰
期 刊 :中国中药杂志 2015年 40卷 15期 页码:2999.
关键词:小叶山葡萄 ;化学成分 ;茋类 ;细胞毒活性 ;
Keywords:Vitis thunbergii var.taiwaniana , chemical constituents , stilbene , cytotoxicity ,
摘 要 :该文对小叶山葡萄地上部分化学成分进行了研究,运用硅胶柱色谱,ODS中低压柱色谱,Sephadex LH-20凝胶柱色谱,分析型和制备型HPLC从小叶山葡萄60%乙醇提取物大孔树脂95%乙醇-水洗脱部位分离得到12个化合物,利用高分辨质谱,核磁共振等波谱手段鉴定其结构分别为:(1 )betulinic acid,(2 )2, 2, 2‘-bis(4-hydroxyphenyl)propane bis(2,3-epoxypropyl)ether,(3 )eriodictyol,(4 )trans-ε-viniferin,(5 )(+)-cis-ε-viniferin,(6 )kobophenol A,(7 )ampelopsin A,(8 )nepalenhenol,(9 )cis-miyabenol C,(10 )cis-vitisin B,(11 )cis-gnetin H 和(12 )(+)-hopeaphenol。化合物 2,5,6,8,9,10,11 均为首次从为葡萄属中分离得到的化合物,化合物 3,7,12 为首次从小叶山葡萄中分离得到的化合物。在作用浓度为50 μmol·L-1 下,化合物 6,7 和11 对MCF-7(乳腺癌细胞株)具有较明显的体外生长抑制作用,其抑制率分别为66.58%,57.16%,52.84%。
Abstract:This article studied the chemical constituents from the aerial part of Vitis thunbergii var.taiwaniana. The 60% ethanol extract was eluted with 95% ethanol though HP-20 macroporous adsorption resin column. 12 compounds, including(1 )betulinic acid,(2 )2, 2, 2‘-bis(4-hydroxyphenyl)propane bis(2, 3-epoxypropyl)ether,(3 )eriodictyol,(4 )trans-ε-viniferin,(5 )(+)-cis-ε-viniferin,(6 )kobophenol A,(7 )ampelopsin A,(8 )nepalensinol B,(9 )cis-miyabenol C,(10 )cis-vitisin B,(11 )cis-gnetin H and(12 )(+)-hopeaphenol, were separated by using normal phase silica gel, ODS, Sephdadex LH-20 column chromatographies and semi-preparative or preparative HPLC. Compounds 2, 5, 6, 8, 9, 10, 11 were separated from the genus Vitis for the first time and compounds 3, 7, 12 were separated from Vitis thunbergii var.taiwaniana for the first time. At a concentration of 50 μmol·L-1 , compound 6, 7 and 11 showed strong cytotoxicity against MCF-7 cell lines with the inhibition rate of 66.58%, 57.16%, 52.84%, respectively.
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