Abstract:Eleven compounds were isolated from the leaves of Epimedium pubescens by means of various chromatographic techniques such as silica gel, MCI, Sephadex LH-20 and preparative HPLC. Their structures were identified as anhydroicaritin(1),icarisideⅡ(2),2‘‘‘-O-rhamonosyl-icarisideⅡ(3),desmethylanhydroicaritin(4), baohuosaideⅡ(5), epimedokoreanin B(6),acuminatin(7),tricin(8), kaempferol(9), daidzein(10) and 4-hydroxy ethyl benzoate(11) on the basis of physicochemical properties and spectroscopic data analysis. Among them, compound 11 was isolated from Epimedium species for the first time, and other compounds were obtained from this plant for the first time.