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作 者 ：赵小亮,王智民,马小军,荆文光,刘安

期 刊 ：中国中药杂志 2013年 38卷 5期 页码：703.

关键词：荷叶；化学成分；黄酮；体外淀粉样蛋白(β-amyloid；Aβ)抑制活性；

Keywords:leaves of Nelumbo nucifera, chemical constituents, flavonoids, beta amyloid (1-42) inhibitory activity,

摘 要 ：研究植物莲Nelumbo nucifera干燥叶的化学成分,为进一步开发利用荷叶提供依据。分离得到27个化合物,分别为10-二十八烷醇(1)、β-谷甾醇(2)、1-十一烷醇(3)、1-二十烷醇(4)、胡萝卜苷(5)、6‘-hydroxy-4,4‘-dimethoxychalcone(6)、1-羟基-3,7,8-三甲氧基 NFDE8 酮(7)、鼠李素-3-O-β-D-吡喃葡萄糖苷(8)、chrysoeriol-7-O-β-D-glucoside(9)、槲皮素-3-O-β-D-吡喃葡萄糖苷(10)、槲皮素-3-O-α-L-吡喃鼠李糖苷(11)、金丝桃苷(12)、槲皮素-3-O-芸香糖苷(13)、紫云英苷(14)、异鼠李素-3-O-α-L-鼠李糖-(1→6)-(15)、异鼠李素-3-O-α-D-来苏糖-(1→2)-β-D-葡萄糖苷(16)、异鼠李素-3-O-β-D-葡萄糖苷(17)、异鼠李素-3-O-α-L-鼠李糖-(1→6)-β-D-葡萄糖苷(18)、槲皮素(19)、山柰酚(20)、去氢荷叶碱(21)、莲碱(22)、stigmast-7-en-3-O-β-D-glucopyranoside(23)、stigmast-7-en-3β-ol(24)、邻二羟基苯(25)、alangionoside L(26)、icariside B₂ (27)。化合物1, 6～8, 24, 25为首次从该植物中分离得到,化合物15～18为首次从荷叶中分离得到。经体外活性筛选,发现化合物6, 8, 10, 11, 13, 15对体外淀粉样蛋白(β-amyloid, Aβ)具抑制活性,抑制率分别为(63.99±24.29)%,(79.61±4.49)%,(49.96±12.61)%,(101.19±8.19)%,(88.41±6.76)%,(72.48±8.97)%,其中有5个化合物为黄酮类化合物。

Abstract:To study the chemical constituents, twenty-seven compounds were isolated from the 70% ethanol extract from leaves of Nelumbo nucifera by modern chromatographic techniques. Their structures were identified as 10-octacosanol (1), β-sitosterol (2), 1-undecanol (3), 1-eicosanol (4), daucosterol (5), 6‘-hydroxy-4,4‘-dimethoxychalcone (6), 3,7,8-trimethoxy-1-hydroxy-xanthone (7), rhamnetin-3-O-β-D-glucopyranoside (8), chrysoeriol-7-O-β-D-glucoside (9), quercetin-3-O-β-D-glucopyranoside (10), quercetin-3-O-α-L-rhamnopyranosyl (11), hyperoside(12), quercetin-3-O-rutinoside (13), astragalin (14), isorhamnetin-3-O-α-L-rhamnopyranosyl-(1→6)- (15), isorhamnetin-3-O-α-D-lyxopyranosyl-(1→2)-β-D-glucopyranoside (16), isorhamnetin-3-O-β-D-glucopyranoside (17), isorhamnetin-3-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside (18), quercetin (19), kaempferol (20), dehydronuciferine (21), roemerine (22), stigmast-7-en-3-O-β-D-glucopyranoside (23), stigmast-7-en-3β-ol (24), and benzene-1,2-diol (25) on the basis of spectral data analysis. Compounds 1, 6, 7, 8, 24 and 25 were isolated from this plant for the first time, and compounds 15-18 were isolated from the leaves for the first time. Compounds 6, 8, 10, 11, 13 and 15 showed inhibitory activities against beta amyloid (1-42) by A-beta aggregation method with inhibition rates of (63.99±24.29)%, (79.61±4.49)%, (49.96±12.61)%, (101.19±8.19)%, (88.41±6.76)% and (72.48±8.97)%, respectively.

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