免费文献传递   相关文献

Structures and bioactivity of polysaccharides from Isatidis Radix

板蓝根多糖的结构特征及活性研究



全 文 :#wxuyw
#
VWê™U[01¯XGY›ƒ„
}ù~
P#W
#

P#W
!
#
.)n
P
#
\‚‹
P#W
#
Ýãã
P
#
.“
P# W
!Ph
©þ8HKA¯
#
œ^ ©þ
WPMM^X% Wh
œ^78KIYÿ89:;
#
œ^ ©þ
WMMMW_
$
†;
%!

&
ƒ„Ñ6‹VWê™U[\]01ÑãÌY›

ˆ‰
.
VWêmµŽñ¾ËŒ{‰64l!
&™U
&
“
›—éîa{‰”
$.8?8%
%Ô¿Œ|™U[lѹŒjA6Y·
!
Ë
,S!:3!><3!
—ò@c\Ñ
1I2#
ZQ”ƒ„
¿™U[\]01
!<@@
”1%¿™UAóô¨©[kĪ«E›

Š‹
.
64l\&bVWê™U
=!7!!R!-!
¿™U[
¹ŒjA6Y·6Fê
pW MMM VR$!P [L[hP!P O^Wh[!_LLhX!PPh[ VR$
VWê™U
=
‚&xbcdUÙÚ
!
™U
-
[HU
Ùڂ&êbcdUÑ7rU
!
™U
7!!R
ŒxdeUÙÚ

™U
=!7!!R!-
‚&ê#U¤Š

™U
=l-
rŒ]
P

W!P

O!P

^!P

X
™ U¤Š[E|…}

VWê™U
!R!-!
Aóô¨©[kĪ«!(
$1:%
6Fê
LhOP!^h[X!LhP[
¼R
K–ª[¹õ=8E›

ŠŒ
.
VWê™U
=l-
êp5±÷ÉÌr64&b[6ݙU

$
Ž
%!
VWê
&
™U
&
¹õ=8
&
016‹
$
8$f
%!WMPPMOMOMMO
$
‘’Eq
%!
­æléö]stuv
$NPM[OMWO%&
­æäKØDE
-stuv
$ WMM_OWO[PWMMPL %&
£@R—°lés tu v
$M_C97OXMMMX%
$
“„>”
%!
!

!
äö
!
DEã·Â
!@(&.$MWL% NLNPPLPW!
-;I$&.&K$)*qNPNW\PXOhG/I
$
>”Ÿ 
%!
Êt÷
!
DE
!
øäö
!
±×rÇG8éÇÌ\]ä]
öƒNE
!@(&.$MWL% NLNP^MOL!-;I$&.#(q&E\PXOhG/I
!!
VWêÇÁr™UÚ6ãÇ·[
_hONg l
WWhM_g
P(
!
]!ã[mµŽñÜÛNÞruØ6
Å@®

VWê™UA6v[þž¹õѨ©¹õ
Œè|Ô[=8E›
W(

VWê™U*Z·~R
—
$B81%
kIJA[
>J;
$
7
i
R>=
0ÄY›[3
—
!
±fÇÈ
>J;
$
7
¨Äi¹õ
#
øùè‰[|F
J[s@¼k
O(

¹õÞg[µ—
!
]bÐR[*
ÿ_³‰Š›E›
!
*gMVWêbÐRÒé[‚
&ÝÜg|

@f
!
]ˆ´Ñ/(VWêY›™U
ØI[s©_
!
64l\>6‹Œ|jA6Y·
™U[ÙÚÑ01
!
]QЉ.VWêbÐR[Ì
Ys©ÑÄ¥¬›ÇÁ™¬…T’èi&@Ø

!
ÁÂ
—éžj{‰ß
$
de
7<;PM%&
rIöx
1%à
$-B1R !=&2(G# LMM%&@1C;*(&?LMMM 8aKP
îaš¿{‰Œ
$
¥¤
@T1T.
–$ä]
%&.AU(%0&;
>.W;L4I
6s{‰Œ
$
uE¦¬¯6‹ßàè#–
$
%
de
,SW^MP
¡®6II.
&>G/&(2:3PMM
¬®I‰ß
&
*úЛß
$73,C-3=;OMM%& @.^;
WMM
âûœuXv
$
ýùüNö]ßàè#–$
%&
1a;9;PJ
â oNEu
$
@Go\áÝå
%&8/E(%;
E$D(O^M
â2·¹õ1%ß
$
­dÎú.ûå
%&
OPPP
â
T
W
ý0
$@#(%I/%& _X
˜õPV
$/%))*%
6vóô¨©`
=);P
xröb_&¨©²µ
o

¿ HUA“]Œê
J&4V$
ä]
38<:;PX^M
õ
PsêÒ­
?:7T
–$ä]
!
Šã6þQó
J1

lrÒęïGÏ&ãÌN.è#–$
!
–Ùsÿ„
Ôj
<@@#
ö¿t2
@AU0)
ê
=I%(0G/
ä]
!1(U#$;
5(K?;PMM!R(K2%$)@;WMMM!@;PXMM!@;ONM!@;[M!@;^M!
@;PM
ê
1*I$
–$ä]
!
ªpçیê6‹l

VWêÇÁäQê£@E!
!
ËýùrÏÇ
u]ÕÔäƒyHs#äöÕÔêÿ8IöÉÌ$
W
;4.!0403?0:[ÇLê

#
…”
#]!
VWê™U[64ilVWêÇÁ
N
ý
#X
ý·mµ#
W
5
!
mµ#
žM<¢0ãÇ<
P */IB
QP
!
aЏŽ0

[Mg!
&bñêVWêK™UØI
&

UØIËmQ¾
!
aŽ6%ñ
!
&b
LMg!
[Mg
Žñ™UØI
!
Ë
1(U#$5(K?WMM
|å{‰6
4
!
„™
;
?@”1C¿&6GI
!
Ä>jZGH
{[&6
!
Ë
1(U#$5(K?WMM
¢jl!
—…ÇL
!
/_[PW/
{
OX
ï{
PX
•
WMPP
÷
N
²
!!!!!! ! ! ! ! ! !!!! ! ! ! !
S/&hOX! :004(!PX
=4*402! WMPP
&b¿VWêl\™U
=!7!!R!-
#]#
VWê™U[lGjA6Y·%Ô
“›—éîaš¿{‰”
$.8?8%
AVWê
™U
=!7!!R!-
8ÉlGjA6Y·[%
Ô
!
{‰;qêîaš¿{‰Œ
@1C;*(&?LMMM
8aKP!
lmê&Êj
!=&2(G# LMM
rIöx1%
à1%
!
&^û®
PPM j R(K2%$)
A“]
@;WMMM!@;PXMM!@;ONM!@;[M!@;^M!@;PM
GVWê™
Uƒ]6F8ƒ
!
%ÔA-7š
!
jA6Y·A
(_AA-7šÜÀ+|n
!
.P¿™Uƒ][
jA6Y·

#]$
VWê™U[ÙÚ6‹
N(
¿Œ|jA6Y·VWê™Uƒ]Ë
W I/&
/B
QP
[?@mQ
!7$T
O

!

!
@®ñ
!
#
_óžEêoç]ž
P;
deU
#P;
bcdU
#P;
7rU
#P;
µÈU
#P;
•U
#P;
¦U
#$;
v>U‡HU
EêA“]ž

#É>[HUA“]žÑ™UmQž
!
6F
-
MhPL I/&/B
QP
[[@ügܝž(Ü[)
a
?
V_
!
»SŽ
;
…Ž
;
m
$^mLmP%
æ—
!
„z
;
0
„üÙ@u{
!
•–ƒ]ÑHUA“][
36&
6F
G#HUA“]žÑ¿™UmQž
!

.8B
1%
!
•–ƒ]ÑHUA“][A-7š

#]%
VWê™U¡®I‰6‹
#¿Œ|jA6Y·™Uƒ]ÉÚ
MhL */
B
QP
mž
!
›¡®6II.]µx
WMM l^ MM )I
¬­
!
%Ô¡®GHI‰

#]&
VWê™U[¬®I‰6‹
#¿Œ|jA6Y·™Uùþƒ]
MhP I*!
i
C7%
~ÄMr
!
]
^ MMM l^ MM GI
QP
¬­
!
%Ô¬
®GHI‰

#]
VWê™U[
P
.;><3
1%
VWê™Uƒ]
PM I*

R
W
T
êÛ
!73,C;
-3=;OMM
*úЛß%Ô*úЛµ‰

#](
—ò@c\Ñ
1I2#
ZQ
#~
PL I*
™Uƒ]
!
93PÔ
!
’7«
L(
…
”8ɗò@c\Ñ
1I2#
ZQ§¨
!
.P—ò@
¹É·ÑÙ@ãÚ·
&
¬›
.8B
AmQ²&UŽ
8Éԛ

#])
VWê™U¹õY›%Ô
6vóô¨©`
=);P
Ë!ôºA(ãx•[
*+¨©
!
6FÛ¿VWê™U
!
±›A“ÇÌêY
,-™U

]õP0rE¼õP
^N #!<@@
”
X(
¥
{
!
2Àß
^_M )I
Ö%GI
!
.PkĪ«!
(
$1:%
$
0•i†,
$]!
VWê™U[64ilLMg![Mg
Žñ™UØ7Ë
1(U#$5(K?WMM
|
å{‰64
!
&t{.ëVWê™U
=!7!!R!-!
&‹ 6 F ê
MhLLg! Mh^Pg! Mh^Mg! MhW_g!
MhPWg
Ë¡
.8?8
1%
!
¿™UŒºH|AP
{
!
{TU9|\”.PlŒ]
_Xg
_
!
h.
’MŒ|jA6Y·[™UÚ6

$]#
VWê™UjA6Y·[%Ô

W MMM!P XMM!ONM![M!^M!PM VR$
[
R(K2%$)
FJÀ+dÄU[A-7šê…„ÀÑjA6Y
·[A(ꃄÀ
!
ÜÀ+|n
!
&È9….
DYQMhO_L _EnLh[^[ [!/YMh___ P
#VWêl
\™U
=l-
¿
W I*
›
P IB
lmQ
!
%Ô¿ƒ
][A-7š
!
.PVWê™U
=l-
[jA6

!
Ÿ¼
P
¼
P!
VWê™U[jA6Y·
ƒ]
!ZI)
jA6Y·
ZR$
= XhM_L pW MMM MMM
7 XhOWW P [L[ PMM
XhXP[ P O^W [MM
R Xh__M _LL [MM
- PPhNP_ PP [MM
$]$
VWê™U[ÙÚ6‹
VW꿙Uƒ]Ë@mQ
!
ªäÌË
@B
1
%

VWê™U
=
[mQ‚&äÌêbcdU
&
™
U
-
mQ¾è
W
/-
!
6FêbcdUÑ7r
U
&
™U
7!!R
[mQä̌êdeU

¬›
.8B
_[A-7šA¿™UmQäÌ8Éԛ
!
`&bjZ[0•

$]%
VWê™U[¬®I‰6‹
$]%]!
˜gÖ6‹
!
¬®I‰¬­0•Ÿ¼
W!
¿
™Uƒ]]
O XMM lO WMM GI
QP
Öè|ä{
!
ê
T;.
[¥¢›Ê
&O MMM lW NMM GI
QP
Ö[GH{M
;.
[¥¢›Ê“³
!
f
P ^MM lP WMM GI
QP
Yé0[G
H{ÚMx
;.
[D`›Ê“³
!
èºMU¨[¯
X{
P WMM lP MMM GI
QP
(°KR[–ªGHê
;T
[¥¢›Ê
&P [MM GI
QP
”[GH{M
YT
/MNPW/
{
OX
ï{
PX
•
WMPP
÷
N
²
!!!!!! ! ! ! ! ! !!!! ! ! ! !
S/&hOX! :004(!PX
=4*402! WMPP
sÖ[¯X{
!
f
P XMM lP XLM GI
QP
[GH{ÚM ™Um\¾|Ú[

¼
W!
™U[¬®I‰6‹
$
ZGI
QP
= 7 R -
›Êü}
O W_O O WX_ O ONN O OX_ O ^P[ T;.
¥¢›Ê
W _W[ W _OW W _OX W _O^ W _WL
U_
;.
[¥¢›Ê
P [^M P [^^ P [^P P [^W P [^M YT
¥¢›Ê
P XP[ P XMX P XP^ P XWO P XW[
P OX^ P O[P P O[W P O[O P WLW ;.6/%I$2/) Db%$2/) /604*$%
P OW^ P OO^ P OOP P OOL
P MOO P MWP P MP_ P MWM P M[[ ;T
¥¢›Ê
P MXW P P^X P M__ P PMM
P PMM P P^W P P^[
NOW NOM NO[ P;.6/%I$2/) Db%$2/) /6
#
;UA%$)/0($UA%$)/0(%
N_W P;.6/%I$2/) Db%$2/) /6
#
;UA%$)/0(
[L_ [X[ [XW [XP [[L
Ó1U[AP¥¢›Ê
_PN _P_ _WP
Ó1U[AP¥¢›Ê
N[P
–gÓ1[AP¥¢›Ê
$]%]#
U¤Š1â[/Ô
!
_£0•u½
!
VWê
™U
7!!R
x)xÓ1UdeUÙÚ
!
]
N^M GI
QP
”ŒKRGH{
!
M#
;
2sC†E1[
;.
D`
›Ê“³
!
)½*ݏVWê™U
7!!R
#U¤
Šê‚

VWê™U
=
ê‹xÓ1UbcdUÙ
Ú
!
2sE1[ݏi)xÓ1UYZ
!
ê)7«1
#
!
Ùê
;

!
\÷]
N^M GI
QP
”KRGH
{
L(

f§1d‰u½ª]
N_M GI
QP
KRGH
!
”
Ǐê #
;


VWê™U
-
]
N_M!N^M GI
QP
Œ
,.uGH{
!
ªU¤Š1â×m¡
><3
‰/Ô

$]&]$
U*¨F[/Ô
!
VWê™U
7!!R
]
_WM GI
QP
”Œè¬®GH
!
ê
P;
Ó1U*1AP
¥¢›Ê[¯X
!
#
;P;
Ó1U¨[GH{–ª
&
f
[[M GI
QP
[{Úê
P;
Ó1*[AP¥¢›Ê

V
Wê™U
-
[U*1AP¥¢›ÊKR]
N[L
GI
QP
”
!
ê–gÓ1*[¯XGH

$]
VWê™U[
><3
‰6‹
™U[
P
.;><3
‚&›ÝU¤Š[1â

|Ÿ;h
!
™U

P
_Y[nf]!
^hN lLhL!
#
;
1
âÓ1U¤[

P
Y\]I^u
L!
f
;
1âÓ
1U¤[

P
Y[\]I^6
L
±VWê™U
-
[
P
.;><3
‰r*Ÿ
!
‚&E¦xYnfŒu

L!
¼.™U
-
#
;
âU¤Šê‚

$](
—ò@c\Ñ
1I2#
ZQ
¿™Uƒ]˗ò@c!
èYZ.[¹É
!
™U
7!
˗ò@c\¾3èÙ@ãÚ
&
™U
=
¹
Œ7=>Uás¹É—ò@ê
Mh_LX I/&!
><,Ù
@ãÚ
!
h.ª01rZèu·[0ü­sf,0
ƒ­sњü­s
1I2#
ZQ¾
!
‚&äÌêµ
”
!
‘4b™U
=
[‚&HUÙÚêbcdU
!
”
Usš[‚&E|…}ê
P

^
E|

™U
7
¹Œ7=>Uás¹É—ò@ê
PhMXO
I/&!
>ãÚ
MhWNP I/&
Ù@
!
h.01rè
P

X
Š
!
xò@[¹É·ÑÙ@ãÚ·
!
*í01rÆè|
Ô(·[
P

W
J
P

^
Ñ
P

O
U¤Š[Œ]

1I2#
ZQäÌ굔ÑdeU
W
 
!
h.01r¹
è
P

^
Š
!
™U01‚&
P

X!P

W!P

O
E|
ê‚
!
ª•ËuAê
Lm_mL
™U

¹Œ7=>Uás¹É—ò@ê
PhO[^
I/&!
è
Mh^[N I/&
Ù@ãÚ
!
h.Œ]u·
P

X
Š
!
Ë
1I2#
ZQ¾
!
¹èdeUÑ56ŽãÚ
!
h.U
7YŒ]
P

O
Ñ
P

^
U¤Š
!
Ó÷™U01‚&
ê
P

X
Ñ
P

W
E|
!
Ë.P•Ë~ê
PmP
™U
R
˗ò@c\¾¹èÙ@ãÚ
!1I2#
ZQäÌ굔ÑdeU
!
µ½
P

O
Ñ
P

W
E
|ê‚
!
(·•Ë~ê
WmO
™U
-
‚&xbcdUÑ7rU
W
 HUá
sÙÚ
!
—
W
 HU`KR]
1I2#
ZQäÌr
!
h
.
P

O
E|…}]
W
 Usr’èu·Œ]
&
—ò
@èe·¹Éf¹èÙ@|Ú
!
h.7rU
P

W
/PNPW/
{
OX
ï{
PX
•
WMPP
÷
N
²
!!!!!! ! ! ! ! ! !!!! ! ! ! !
S/&hOX! :004(!PX
=4*402! WMPP
E|
!
fbcdU
P

W#P

^
E|’è*g

@
½™U
-

P

O
ŠE|ê‚
!
>Z7Œ]
P

W
Ñ
$
J
%P

^
[E|…}

#])
VWê™U¹õY›%Ô
VWê™U[GIiϛA“Ù•–
!
Ώ
u¡›CE
$oMhMP%&
ªr™U
!R!-
[kĪ
«!(
$1:%
6Fê
LhOP!^h[X!LhP[!
—±›A“
Ù
$
Y,-™U
%
[
1:$^hLL%!
¼.VWê™U
!
R!-
¹õ=8E›Y›–ª
!
Ÿ¼
O
¼
O!
VWê™UAóô¨©[kĪ_éƒ]
Û·
Z*/IB
QP
GI
1:
= PhWLM
PhLMM iMhP^[
P%
OhPO
MhPWL
PhOM[ iMhP[_
P%
Wh[W
7 PhWLM
Mh[MO iMhML^
P%
Ph^[
MhPWL
PhM_^ iMhMNX
P%
WhWN
PhWLM
WhL^^ iMhPP_
P%
LhOP
MhPWL
WhPO_ iMhW^_
P%
^h^X
R PhWLM
WhWNW iMhOLP
P%
^h[X
MhPWL
WhPPL iMhP[[
P%
^h^P
- PhWLM
Wh^NM iMhP[N
P%
LhP[
MhPWL
WhWP^ iMhPWL
P%
^hXW
±›A“
PhWLM
WhPNM iMhPLN
P%
^hLL
MhPWL
WhWNX iMhPLO
P%
^h[[
A“Ù
Mh^NM iMhM^_
!!
?
.
P%
iA“j•
oMhMP
$
†,
U¨Eêã8nÚ[æªFÑ!9F
!
]a9
#
!!
K
#
6#
ÖËFãѹõFã[Æ»‡…T³i
i&E›
[;_(

¤5§¨±VWê[mµØIr6
&>
L
™UÚ6
!
ªr
!
VWê™U
!-!R

u¡[¹õ=8E›
&
VWêY›™U[ÙÚÑ0
1–êj£
!
¤§¨AªÙÚÑ01¯-8É>L
M[ƒ„
!
ªcš01:0ÑY›[j‰›×]A
¾[NEr8|M‰.

$
•ƒ–—
%
P(!
 ma
!
Hºã
!
;.
!
‡
F
üýþ[YZ]Fi–ýþVW
êr™U[Z·%Ô
9(F
{ü<Ïu]]1
! P___! WM
$PM%.[LLh
W(!
Ž–Ï
!
l¹Ú
!
Ò¬?
!
‡
F
VWê™U=8¹õÞg[§¨
ƒ„
9(F
r)Ï0Ä£¤
!P__P! PP$X%.OL[h
O(!
C=¹
!
Hs#
!

!
‡
F
VWê™UA
>J;
$
7
Y›[E›
9(F
ÇÌãÌ÷Ñ
! WMMP! N$L%. W[Xh
^(!
ñ>?
!
ñ¹Ø
!
>@
!
‡
F
AB™U[Ú6GªjA6Y·
ƒ„
9(F
—‡]°\]]1
! P__N! P_$_%.PLPOh
L(!
ñF¡
F
UjÄÌã\ƒ„÷Ñ
<(FW
Â
F
CG
.
Ê£u]
KÂÐ
! WMMO. OXh
X(!
ÈD!
!
EûF
!

F
Ç¥§¨…”]
<(F
øù
.
±Ïâã
KÂÐ
! WMMO. P[Nh
[(!
ß­Î
!
HO#
!
>¤G
!
‡
F
Y›™U[ƒ„8æ
9(F
]
iHNç
! WMMP! W[$[%.^Lh
N(!
4âI
!

!
JF
rǙUƒ„8æ
9(F
r­ÏbÇ]
£¤
!WMMO! WO$X%.OXMh
_(!
Òá}
!
 L
!
…U÷
F
ZQU™UGMU[ƒ„8æ
9(F
Ç
]]1
! WMM^! O_ $PW%. PMWNh
=DGIUDIGAK/CHO0B/UD0S0DF BMNB1FK/UUE/G0HAKMGB.4K/D0H0K:/H0Q
.-BE(
P!W
! B:+$)*
P!W
!
! a=>?./)*&$)
P
! .->9$)E(
P!W
! 1,>R/)*0/)*
P
! a=>?<)*A$)
P!W
$PhN.3B03: %30K,/40!A<=6>03,4,8,?0*03,! N.3B03: WPMM^X! 6>03.&
WhQ0.3:4+ /03,4,7,/1.-/<*,403:! N.3B03: WPMMW_! 6>03.%
!!6OKDG/UD(8O[AUD0SA. @/)D(02*$2(5 2#(G#(IG$&02%4G24%(0$)5 b/$G2D2A/6U/&A0$GG#$%5(06%/I:0$2503$5Kh>ADEBH.
8/&A0$GG#$%5(0E(%((K2%$G2(5 $)5 U4%6(5 bAG/&4I) G#%/I$2/*%$U# $)5 2#(%G#(IG$&02%4G24%(0E(%(5()26(5 bA,S! :3! ><3!
U(%/5G$G5 /K$5$2/) $)5 1I2# 5(*%$5$2/) I(2#/5 $)5 2#(%02I4&$2/) (6(G202/I$G%/U#$*(E(%((D$&4$2(5 bA40)*<@@I(2#/5h
:AKI1D. JD(U/&A0$GG#$%5(0! U/&A0$GG#$%5(=! 7! ! R$)5 -E(%(*/2() $)5 2#(%I/&(G4&$%E(*#20E(%(W MMM! P [L[hP! P O^
Wh[! _LLhX! PPh[ VR$! %(0U(G2D(&Ah8/&A0$GG#$%5(=E$0G/IU/0(5 /6$%$b)/0(! U/&A0$GG#$%5(-E$0G/IU/0(5 /6$%$b)/0($)5
*$&$G2/0(! U/&A0$GG#$%5(07! ! RE(%(G/IU/0(5 /6*&4G/0($)5 P

W! P

O! P

^! P

X &)V$*(0(K02(5 ) U/&A0$GG#$%5(0=;-!
/6=! 7! ! R! -E(%(
%
;G/)6*4%$2/)0h8/&A0$GG#$%5(07! $)5 R0#/E(5 b(2(%b/$G2D2A2#$) U/&A0$GG#$%5(0=$)5 -E2#
02I4&$2/) )5(K$1:% /6LhOP! ^h[X! LhP[h2BCU1IK0BC. JD(U/&A0$GG#$%5(0$%(0(U(%$2(5 6%02&A6%/I:0$2503$5Kh
A^F WBGHK(!:0$2503$5K& U/&A0$GG#$%5(0& II4)/&/*G$&02I4&$2/)& 02%4G24%$&$)$&A00
HB0.!+_%#)RU[U..#+!!!+&
$
˜™$Q
!
£¤¥
%
/WNPW/
{
OX
ï{
PX
•
WMPP
÷
N
²
!!!!!! ! ! ! ! ! !!!! ! ! ! !
S/&hOX! :004(!PX
=4*402! WMPP