作 者 ：汪健红,陆付耳,董慧,龚艳琳,徐丽君,邹欣,陈广
期 刊 ：中国中药杂志 2011年 36卷 16期 页码：2271.
Keywords:Jiaotai pill, compatibility, type 2 diabetes mellitus, rats,
摘 要 ：目的: 探讨不同配伍比例交泰丸对2型糖尿病 (T2DM) 大鼠的治疗作用。 方法: 采用尾静脉注射小剂量链脲佐菌素(streptozotocin, STZ)加高脂高热卡喂养的方法建立大鼠T2DM模型,将大鼠随机分为模型组、交泰丸1组(黄连-肉桂 2∶1)、交泰丸2组(黄连-肉桂 4∶1)、交泰丸3组(黄连-肉桂 10∶1)、二甲双胍组,各治疗组分别给与相应药物灌胃治疗,另设正常对照组。干预治疗8周后观察各组大鼠体重、口服葡萄糖耐量试验(OGTT)、血脂(TC,TG,HDL-C,LDL-C)、血浆肝酶活性(ALT,AST,AKP,γ-GT)、血清脂联素、游离脂肪酸(FFA) 和肝组织病理学的差异。 结果: 与正常组比较,模型组大鼠体重增加,空腹血糖(FPG)、餐后1 h (PG-1 h)和餐后2 h (PG-2 h)升高,TC,TG,LDL-C,FFA,血浆肝酶活性均升高,HDL-C和脂联素水平均下降(P<0.01),肝组织脂肪变性明显;与模型组比较,各治疗组OGTT改善,TC,TG,LDL-C,FFA、血浆肝酶活性均下降,HDL-C和脂联素水平均升高(P<0.05, P<0.01), 肝组织脂肪变性减轻;与二甲双胍组比较,交泰丸1组HDL-C明显升高 (P<0.05), AKP,γ-GT均明显降低(P＜0.05,P＜0.01),交泰丸2组γ-GT降低(P＜0.05),交泰丸3组AST,AKP,γ-GT降低(P＜0.05,P＜0.01);与交泰丸1组比较,交泰丸2组HDL-C降低,AKP升高,交泰丸3组HDL-C降低(P<0.05)。 结论: 不同配伍比例交泰丸对大鼠T2DM都有一定治疗作用,而以交泰丸1组疗效最佳。
Abstract:Objective: To study the effects of different compatibility proportion of Jiaotai pills on treating type 2 diabetes mellitus (T2DM) in rats. Method: The model of type 2 diabetes mellitus in rats was established by injecting streptozotocin from tail vein and feeding with high fat and high caloric diet. Diabetic rats were randomly divided into model group, Jiaotai pill 1 group (Coptidis Rhizoma-cinnamon 2∶1), Jiaotai pill 2 group (Coptidis Rhizoma-cinnamon 4∶1), Jiaotai pill 3 group (Coptidis Rhizoma-cinnamon 10∶1) and metformin group. Rats in different treatment groups were given by corresponding therapy from gastric tube. Meanwhile normal control group was another set. Body weight, oral glucose tolerance test (OGTT), blood lipid level including total cholesterol (TC), triglyceride (TG), high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C), plasma levels of free fatty acid (FFA) and adiponectin, plasma liver enzymes activity(ALT, AST, AKP, γ-GT) and pathological results of liver tissue were determined after eight weeks. Result: Body weight, fasting plasma glucose (FPG), postpradial plasma glucose at one hour (PG-1 h), postpradial blood glucose at two hour (PG-2 h), plasma levels of TC, TG, LDL-C,FFA and liver enzymes activity were all increased in rats of model group compared with those in normal control group. Plasma levels of HDL-C and adiponectin were decreased in model group (P<0.01). Fatty degeneration of hepatocytes was apparent in liver tissues in rats of model group. Compared with model group results of OGTT, blood lipid levels and liver enzymes activity were improved while levels of HDL-C and adiponectin were increased in rats of different treatment groups (P<0.05 or P<0.01). Meanwhile fatty degeneration of hepatocytes was improved in liver tissues in rats of different treatment groups. Compared with metformin group, plasma level of HDL-C was elevated while AKP and γ-GT were decreased significantly in rats of Jiaotai pill 1 group (P<0.05), γ-GT level was decreased significantly in rats of Jiaotai pill 2 group (P<0.05), AST, AKP and γ-GT levels were decreased significantly in rats of Jiaotai pill 3 group (P<0.05). Compared with Jiaotai Pill 1 group, plasma levels of HDL-C was decreased while AKP levels was elevated significantly in rats of Jiaotai pill 2 group, but HDL-C was decreased in rats of Jiaotai pill 3 group(P<0.05). Conclusion: It is suggested that different compatibility proportion of Jiaotai pills are effective on treating type 2 diabetes mellitus in rats. The effect of Jiaotai pill 1 group is better than that of other therapy groups.