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作 者 ：黄鸣清,蒋东旭,罗明俐,李少华,张怡评,江滨,赖小平

期 刊 ：中国中药杂志 2009年 34卷 20期 页码：2633.

关键词：昆明山海棠；肿瘤；活性部位；总生物碱；

Keywords:Tripterygium hypoglaucum, tumor, active part, total alkaloids,

摘 要 ：目的：采用体内外抗肿瘤实验模型，筛选昆明山海棠的抗肿瘤活性部位。方法：采用离子吸附树脂柱层析法、大孔吸附树脂柱层析法，对昆明山海棠醇提物进行分离、纯化；采用MTT法测定昆明山海棠醇提物各分离部位的体外抗肿瘤活性，通过荷瘤小鼠模型考察各部位对S180实体瘤生长的影响。结果：昆明山海棠醇提物、离子树脂部位（IRP）、大孔树脂部位（MRP）均显示出较强的体内外抗肿瘤活性。IRP部位主要为生物碱类成分，其IC₅₀低至29.90 mg·L^-1，IRP 25，50，100 mg·kg^-1剂量组对S180实体瘤的抑瘤率分别为38.10％，50.60％，60.71％。MRP部位的IC₅₀最低为98.56 mg·L^-1，MRP 100,200,400 mg·kg^-1剂量组对S180实体瘤的抑瘤率分别为42.96％,53.57％,63.69％。大孔树脂不吸附的水溶性部位（WSP）未表现出明显的作用。结论：昆明山海棠具有较强的体内外抗肿瘤活性，总生物碱是其抗肿瘤作用的主要活性部位之一。

Abstract:Objective: To screen the anti-tumor active parts from Tripterygium hypoglaucum by anti-tumor experimental model in vivo and in vitro. Method: Ethanol extraction was separated and purified by column chromatography of ion polymeric adsorbent and macroporous adsorptive resins. MTT assay and the inhibition effect to S180 solid tumor were used to detect anti-tumor activity of each separation. Result: There are anti-tumor activities in the ethanol extraction， total alkaloids， and macroporous resin absorption in vivo and vitro. Minimum IC₅ of Total alkaloids was 29.90 mg·L^-1， and S180 solid tumor inhibition ratio of different dose of 25， 50， 100 mg·kg^-1 were 38.10％， 50.60％， 60.71％ respectively. Minimum IC₅₀ of macroporous resin absorption was 98.56 mg·L^-1， and S180 solid tumor inhibition ratio of different dose of 100， 200， 400 mg·kg^-1 were 42.96％， 53.57％， 63.79％ respectively. Water solubility position had no effect in vivo and vitro. Conclusion: T. hypoglaucum has fine anti-tumor activity in vivo and in vitro， and total alkaloids are the main part of anti-tumor active part.

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