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作 者 ：张丽,赵宁,王蓉,李林

期 刊 ：中国中药杂志 2009年 34卷 12期 页码：1557.

关键词：脊髓；老化；神经营养因子；参乌胶囊；二苯乙烯苷；

Keywords:spinal cord, aging, neurotrophic factors, Shenwu capsule, tetrahydroxystilbene glucoside,

摘 要 ：目的：观察中药新复方参乌胶囊及其有效成分何首乌二苯乙烯苷对老年大鼠脊髓腰节段神经营养因子表达的影响。方法：雄性SD大鼠，分为青年组(6月龄)、老年组(24月龄)、老年+参乌胶囊小剂量(0.8 g·kg-1)、参乌胶囊大剂量(1.6 g·kg-1)、二苯乙烯苷小剂量(0.03 g·kg-1)、二苯乙烯苷大剂量(0.06 g·kg-1)6组。应用Western blot方法检测大鼠脊髓腰节段神经生长因子(NGF)、脑源性神经营养因子(BDNF)、胶质细胞源性神经营养因子(GDNF)表达。结果：与青年组比较，老年组大鼠脊髓腰节段NGF，BDNF，GDNF的表达量明显减低(P<0.05，P<0.01)；灌胃给药3个月，参乌胶囊可显著增加老年大鼠脊髓腰节段的神经营养因子NGF，BDNF，GDNF表达(P<0.05，P<0.01)，二苯乙烯苷可明显增加老年大鼠脊髓腰节段GDNF表达(P<0.05)。结论：参乌胶囊及二苯乙烯苷具有提高老年大鼠脊髓腰节段表达神经营养因子的作用，提示该药可能对延缓脊髓神经细胞老化具有潜在的应用价值。

Abstract:Objective: To investigate the effects of Shenwu capsule (SW) and its component tetrahydroxystilbene glucoside (TSG) on the expression of neurotrophic factors in lumbar spinal cord of aged rats. Method: SD rats were divided into 6 groups, including 6 months old group, 24 months old group, 24 months old + low dose SW (0.8 g·kg-1), high dose SW (1.6 g·kg-1), low dose TSG (0.03 g·kg-1) and high dose TSG (0.06 g·kg-1). Western blot was used to detect the expression of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), gliacyte-derived neurotrophic factor (GDNF) in the lumbar spinal cord of rats. Result: Compared with 6-month-old young rats, the expression of NGF, BDNF and GDNF in the lumbar spinal cord was significantly decreased in the 24 month old rats (P<0.05, P<0.01). Intragastric administration of SW for 3 months obviously enhanced the expression of NGF, BDNF and GDNF in the lumbar spinal cord of aged rats (P<0.05, P<0.01), and TSG significantly enhanced the expression of GDNF (P<0.05). Conclusion: SW capsule and its component TSG elevated the expression of neurotrophic factors in the lumbar spinal cord of aged rats, suggesting that these drugs may retard aging process of the spinal cord.

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