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作 者 ：秦峰,王长松,晋光荣,花菊兰

期 刊 ：中国中药杂志 2009年 34卷 12期 页码：1566.

关键词：犀角地黄汤；脑出血；大鼠 Bcl-2；caspase-3；TNF-α；IL-6；

Keywords:Xijiao Dihuang decoction, intracerebral hemorrhage, rat Bcl-2, caspase-3, TNF-α, IL-6,

摘 要 ：目的：观察犀角地黄汤对大鼠脑出血急性期脑组织Bcl-2 、半胱氨酰天冬氨酸特异性蛋白酶-3(caspase-3)、血肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)表达的影响。方法：以自体血制作大鼠脑出血模型，采用免疫组织化学和放射免疫法检测大鼠脑出血急性期脑组织Bcl-2，caspase-3阳性细胞数，血TNF-α，IL-6的表达。结果：造模后72 h，模型组caspase-3阳性细胞数、TNF-α，IL-6的表达明显高于正常组、假手术组；犀角地黄汤各剂量组caspase-3阳性细胞数、TNF-α及IL-6的表达明显低于模型组，Bcl-2阳性细胞数明显高于模型组。结论：犀角地黄汤可能通过减少细胞凋亡、抑制炎症，对脑出血后的继发性神经元损伤起保护作用。

Abstract:Objective: To study the effects of xijiao dihuang decoction on the expressions of Bcl-2 and caspase-3 protein positive cells in brain tissue, TNF-α and IL-6 in blood after acute intracerebral hemorrhage in rat. Method: The experimental ICH model was established by injection of non-heparinized autologous arterial blood into the right basal ganglia. Immunohistochemistry and radioimmunity methods were used to detect expressions of Bcl-2 and caspase-3 protein positive cells in brain tissue, TNF-α and IL-6 in blood. Result: After ICH, caspase-3 protein positive cells in brain tissue, TNF-α and IL-6 in blood increased obviously in the model group. caspase-3 protein positive cells, TNF-α and IL-6 were significantly reduced by treatment with Xijiao Dihuang decoction, on the contrary, Bcl-2 protein positive cells in brain tissue increased markedly. Conclusion: Xijiao Dihuang decoction can pretect nerve cell by reducing the expression of caspase-3， TNF-α and IL-6，increasing the expression of Bcl-2 after ICH in hemorrhagic brain damage.

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