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期 刊 ：中国中药杂志 2006年 31卷 18期 页码：1526.

关键词：黄连解毒汤；含药血清；抗肿瘤作用；

Keywords:Huanglian Jiedu decoction, serum containing Huanglian Jiedu decoction, antitumor activity,

摘 要 ：目的:对比黄连解毒汤及其含药血清的抗肿瘤作用及其化学成分的异同。方法:采用HPLC梯度洗脱对比分析黄连解毒汤及其含药血清化学成分的异同。并用MTT方法比较黄连解毒汤及其含药血清对人肝癌Bel-7402、人小细胞肺癌NC I-H446 2种瘤株的抑制作用。结果:发现10个来源于复方的原型成分,几个代谢产物,同时一些原方剂中的主要成分并未在血清中出现。黄连解毒汤及其含药血清对2种瘤株都有显著的抑制增殖作用,并呈量效依赖关系,但抑制程度不同。黄连解毒汤对人肝癌Bel-7402相对更敏感(Bel-7402的IC₅₀为185.34 mg.L^-1,NC I-H446的IC₅₀为264.68 mg.L-1),黄连解毒汤含药血清各剂量组对2种瘤株的抑制程度非常相似。结论:黄连解毒汤及其含药血清对2种瘤株抑制程度变化的原因可能是黄连解毒汤中各主要成分吸收入血后含量变化造成的。对其进行深入的研究,将有助于阐明黄连解毒汤抗肿瘤的有效成分及作用机制。

Abstract:Objective: To make a comparison between the antitumor effect and the chemical constituents of Huanglian Jiedu decoction(HLJDT) and that of serum containing HLJDT.Method: Based on the established chromatographic fingerprint of HLJDT,analysis and comparison were made between the HPLC fingerprints of rat serum samples obtained after orally taking HLJDT and those of control rat serum samples.The different effects on NCI-H446 and Bel-74024 cancer cells from human were investigated in vitro using HLJDT and its serum.The inhibitory effects of HLJDT and its serum were observed by MTT assay.Result: Ten compounds of HLJDT and some metabolites were detected after oral administration of HLJDT,and however some main compounds of HLJDT were not detected in serum.Both HLJDT and its serum in different dosage groups could inhibit the proliferation of NCI-H446 and Bel-7402 cancer cells from human in a dose-dependent manner,but inhibitory grade was different in the two cancer cell lines.HLJDT had more inhibitory effect on Bel-7402 than on NCI-H446,on the other hand serum containing HLJDT had the same inhibitory effect on Bel-7402 and NCI-H446.Conclusion: The reason for inhibitory grade change was that the proportion of concentration of many compounds in serum containing HLJDT was different to that in HLJDT,which should be subject to thorough investigation so as to illuminate the pharmacology and active mechanism of HLJDT.

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