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期 刊 ：中国中药杂志 2006年 31卷 03期 页码：200.

关键词：蒲黄；蒲黄炭；气相色谱-质谱联用；

Keywords:Pollen Typhae, Pollen Typhae carbonistatus, GC-MS,

摘 要 ：目的:探讨蒲黄炒炭前后亲脂性成分的变化。方法:将生蒲黄和蒲黄炭分别用石油醚(60～90℃)连续回流提取,提取物甲酯化后进行气相色谱-质谱联用(GC-MS)分析,并利用NIST谱库检索鉴定化合物,用归一化法计算各化合物的相对百分含量。结果:从生蒲黄和蒲黄炭石油醚提取物中鉴定了32个化合物,其中相同化合物20个、不同化合物各6个。在相同化合物中,炮制后相对百分含量显著增加的有3-甲基-2-丁烯酸-2-苯乙基酯、己二酸二甲酯、邻苯二甲酸二甲酯、邻苯二甲酸二乙酯、二苯胺、癸二酸二甲酯、邻苯二甲酸甲基乙基酯、邻苯二甲酸甲基-2-乙基己基酯、邻苯二甲酸二异辛酯等,炮制后相对百分含量显著降低的有壬二酸二甲酯、邻苯二甲酸二异丁酯、3,5-二烯豆甾烷等;在不同化合物中,生蒲黄有而在蒲黄炭中没有检测到的化合物是8-羟基辛酸甲酯、9-羟基壬酸甲酯、10-十八碳烯酸甲酯、间羟基肉桂酸甲酯、3-[4-(乙酰氧基)-3-甲氧基苯基]-2-丙烯酸甲酯、11-十八碳烯酸甲酯等,蒲黄炭有而生蒲黄中没有检测到的化合物是3-羟基-螺甾-8-烯-11-酮、氢化肉桂酸甲酯、2,4-二甲基己二酸、2,4-二叔丁基苯酚、十一碳二酸二甲酯、9,10-二羟基硬脂酸甲酯等。结论:蒲黄炒炭前后亲脂性成分种类和含量都有一定程度的变化,但是化合物类型变化不大。

Abstract:Objective: To study the changes of the fatty components of Pollen Typhae before and after being carbonized.Method:Pollen Typhae and Pollen Typhae carbonisatus were extracted with petroleum ether(60-90 ℃) respectively.The two kinds of extracts were analyzed by GC-MS after saponificated and methanolized,and their constituents were searched through NIST.The contents of the constituents were determined by method of normalization.Result: Either in Pollen Typhae or in Pollen Typhae carbonisatus,32 components were identified,among which 20 components were the same and 6 were different respectively.Among the same components,the relative contents of 3-methyl-2-butenoic acid-2-phenylethyl ester,hexanedioic acid-dimethyl ester,dimethyl phthalate,diethyl phthalate,diphenylamine,sebacic acid dimethyl ester,1,2-benzenedicarboxylic acid,ethyl methyl ester,methyl-2-ethylhexyl phthalate and diisooctyl phthalate etc.increased obviously,and the relative contents of nonanedioic acid-dimethyl ester,diisobutyl phthalate and stigmastan-3,5-dien etc.decreased greatly.Among the different components,8-hydroxy-octanoic acid-methyl ester,9-hydroxy-nonanoic acid-methyl ester,10-octadecenoic acid-methyl ester,m-hydroxycinnamic acid-methyl ester,3-[4-(acetyloxy)-3-methoxyphenyl]2-propenoic acid-methyl ester and 11-octadecenoic acid-methyl ester were detected in Pollen Typhae,3-hydroxyspirost-8-en-11-one,benzenepropanoic acid-methyl ester,2,4-dimethylhexanedioic acid;2,4-bis(1,1-dimethylethyl)-phenol,undecanedioic acid-dimethyl ester and 9,10-dihydroxy-octadecanoic acid-methyl ester were detected in Pollen Typhae carbonistatus.Conclusion: The species and contents of the fatty components in Pollen Typhae changed before and after being carbonized,but their chemical types didn‘t change too much.

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