Research for dependablity of administration of platycodi radix in Tianwang Buxinwan decoction with change of brain inhibitive neurotransmitte in rats by microdialysis
Abstract:Objective: To study the effects of Tianwang Buxinwan decoction on the contents of amino acids neurotransmitters in corpus striatum of rats to implicate the mechanism of Tianwang Buxinwan promoting and Improving sleeping. Method: Contents of two amino acids neurotransmitters in corpus striatum of rats were prepared by microdialysis technology and were determined by HPLC which involved pre-column derivation with orthophthaladehyde, recersed-phase gradient elution and fluorescence detection. Result: In the experimental separation condition, Tianwang Buxinwan seemed do not influence three kinds of contents of amino acids neurotransmiters (glutamic acid, glycin, aspartic acid), but TBW seemed increase the content of γ-GABA in corpus striatum of rats. Conclusion: The effects of Tianwang Buxinwan to relieve uneasiness may relate with the inhibitory amino acids γ-GABA. Tianwang Buxinwan may promote increasing the content of γ-GABA. This discovery may be helpful for the deep study of related mechanism of Tianwang Buxinwan.