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期 刊 ：中国中药杂志 2007年 32卷 18期 页码：1925.

关键词：TFG；心肌细胞；缺氧/复氧模型；TNF-α；Fas/FasL蛋白；

Keywords:TFG, Cardiomyocyte, H/R model, TNF-α, Fas/FasL proteins,

摘 要 ：目的：探讨绞股蓝总黄酮(TFG)对培养的缺氧/复氧(H/R)乳鼠心肌细胞肿瘤坏死因子(TNF-α)浓度,凋亡相关基因Fas/FasL蛋白表达的影响。方法：利用原代培养的SD大鼠乳鼠心肌细胞建立心肌细胞H/R模型,实验分6组：①对照组；②H/R组；③15 mg·L^-1 TFG+H/R组；④45 mg·L^-1 TFG+H/R组；⑤105 mg·L^-1 TFG+H/R组；⑥105 mg·L^-1 TFG对照组。用ELISA法测定心肌细胞TNF-α,免疫组化法检测Fas/FasL蛋白表达。结果：H/R后,心肌细胞Fas/FasL蛋白阳性表达指数(PEI)显著高于对照,TFG组PEI 低于(H/R)组；TFG能抑制心肌细胞产生TNF-α。结论：H/R时凋亡相关基因Fas及其配体FasL蛋白表达增强,TFG对H/R心肌具有保护作用,其机制与减少TNF-α的生成,下调Fas/FasL蛋白表达,减少凋亡有关。

Abstract:Objective: To study the effect of total flaveos of Gymostemma pentaphyllum on the protein expression of apoptosis-associated Fas/FasL gene and tumor necrosis factor-α (TNF-α) concentration in cultured neonatal rat cardiomyocytes with hypoxia-reoxygenation (H/R). Method: A cultured primary neonatal rat cardiomyocytes model with H/R was erected, experiments were divided into six groups, ①control group, ②H/R group, ③15 mg·L^-1 TFG plus H/R group, ④45 mg·L^-1 TFG plus H/R group, ⑤105 mg·L^-1 TFG plus H/R group, ⑥105 mg·L^-1 TFG group. TNF-αconcentration in cultured cardiomyocytes with H/R, was determined by ELISA method, the protein expression of Fas/FasL genes were estimated by immunohisto-chemistry. Result: After cardiomyocytes were made with H/R, Compared with control group, the positive expression index (PEI) of Fas/FasL proteins in cardiomyocytes increased significantly, Compared with H/R groups, the PEI of Fas/FasL proteins were lower significantly in groups with different dosages of TFG (P<0.05). TFG inhibited the secretion of TNF-α from myocardial cells and increased the survival rate of myocardial cells. Conclusion: The protein expression of apoptosis-associated Fas/FasL genes increased during H/R. The TFG can protect myocardium against H/R injury by decreasing the production of TNF-α, downregulating the protein expression of Fas/FasL genes, and then inhibiting myocyte apoptosis.

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