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期 刊 ：中国中药杂志 2007年 32卷 12期 页码：1175.

关键词：盾叶木；化学成分；巨噬细胞TNF-α分泌抑制作用；

Keywords:Macaranga adenantha, chemical constituents, TNF-α secretion inhibitory activity,

摘 要 ：目的：研究盾叶木Macaranga adenantha枝的化学成分并进行小鼠腹腔巨噬细胞TNF-α分泌抑制活性筛选。方法：用各种硅胶和凝胶色谱分离化合物,用波谱学方法鉴定结构；用MTT法测定化合物对小鼠腹腔巨噬细胞TNF-α分泌抑制活性。结果：从盾叶木枝的乙醇提取物中分离得到10个化合物,分别鉴定为cleomiscosin A(1)、cleomiscosin B(2)、鞣花酸-4-O-α-D-吡喃鼠李糖苷(3)、鞣花酸-4-O-β-D-吡喃木糖苷(4)、香草酸(5)、(24R)-豆甾-4-烯-3-酮(6)、(24R)-豆甾-3,6-二酮(7)、(24R)-6β-羟基-豆甾-4-烯-3-酮(8)、胡萝卜苷(9)和β-谷甾醇葡萄糖苷-6′-O-十七烷酸酯(10)；在10 μmol·L^-1浓度下,化合物1,3和4对小鼠腹腔巨噬细胞TNF-α分泌的抑制率分别为57.4%,57.0%和64.4%。结论：化合物10为首次从大戟科中分离得到,化合物1～10均为首次从该属植物中得。化合物1,3和4对小鼠腹腔巨噬细胞TNF-α分泌表现出较强的抑制作用。

Abstract:Objective: To investigate the chemical constituents of the brach of Macaranga adenantha, and evaluate their TNF-α inhibitory activity. Method: The chemical conshifuents were isolated and purified by chromatographic methods. Structures of the compounds were identified by spectroscopic methods. The TNF-α secretion inhibitory activity of the mouse peritoneal macrophages was evaluated by MTT methods. Result: Ten compounds were isolated and their structures were identified as: cleomiscosin A (1), cleomiscosin B (2), ellagic acid 4-O-α-D-rhamnopyranside (3), ellagic acid 4-O-β-D-xylopyranoside (4), vanillic acid (5), (24R)-stigmast-4-en-3-one (6), (24R)-stigmast-3, 6-dione (7), (24R)-6β-hydroxy-stigmast-4-en-3-one (8), daucosterol (9), β-sitosteryl glucoside-6′-O-heptadecoicate (10). At a concentration of 10 μmol·L^-1, compounds 1, 3 and 4 showed inhibitory activity to TNF-α secretion of the mouse peritoneal macrophages with the inhibitory rates of 57.0%, 64.4%, and 57.4%, respectively. Conclusion: All compounds were isolated from genus Macaranga for the first time. Compounds 1, 3, and 4 were active against TNF-α secretion of the mouse peritoneal macrophages.

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