摘 要 ：目的:通过观察黄芪促进橄榄小脑变性大鼠胰岛素样生长因子-1(IGF-1)的表达,探讨了黄芪对橄榄小脑变性大鼠的神经保护作用。方法:采用神经毒性物质3-acetylpyrid ine建立橄榄小脑变性大鼠模型,运用免疫组化法分析Calb ind in D-28K蛋白表达、E lisa法分析血清IGF-1水平、RT-PCR法分析IGF-1 mRNA表达,探讨了黄芪对橄榄小脑变性大鼠下橄榄核神经元存活率及IGF-1表达方面的影响。结果:黄芪能显著提高橄榄小脑变性大鼠血清IGF-1水平和小脑IGF-1 mRNA的表达,有效提高下橄榄核神经元的存活率,对橄榄小脑变性大鼠运动协调能力具有一定的改善作用。结论:黄芪能显著提高橄榄小脑变性大鼠IGF-1的分泌与表达,并对下橄榄核神经元具有保护作用。
Abstract:Objective: To observe the effect of Astragalus membranaceus(AM) on insulin-like growth factor 1(IGF-1) expression in a rat model of olivo-cerebellar degeneration and assess the neuroprotective actions of AM meanwhile.Method: Rats model of olivo-cerebellar degeneration was established by using 3-acetylpyridine.The effect of AM on the expression of Calbindin D-28K in inferior olive(IO) neurons by immunohistochemistry,the serum IGF-1 level by Elisa,the IGF-1 mRNA level in the cerebellum by RT-PCR were detected respectively.Result: AM effectively improve the serum IGF-1 level,Cerebellar IGF-1 mRNA level and the survival of the IO neurons in a rat model of olivo-cerebellar degeneration,even at a lower dose(9 g·kg-1),and the effect was in a dose-dependent manner.Conclusion: AM could effectively upregulate the IGF-1 expression in the rat model of olivo-cerebellar degeneration,and have neuroprotective effect on IO neurons.