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期 刊 ：中国中药杂志 2007年 32卷 13期 页码：1317.

关键词：白藜芦醇；离体心房肌；心率；收缩力；钾通道；

Keywords:resvaratrol, isolated guinea pig atriun, heart rate (HR), contractile force (CF), potassium channels,

摘 要 ：目的：观察白藜芦醇(resvaratrol,Res)对豚鼠离体心肌收缩力(contraction force,CF)和窦房结功能的影响。方法：测定Res对离体豚鼠心房肌CF和心率(heart rate,HR)影响的量效曲线,并研究不同类型钾通道阻断剂在Res对心脏负性变时变力作用的影响。结果：Res可减慢心率(P<0.05),减弱心肌CF (P<0.05)；与Res组相比,ATP敏感性钾通道(K_ATP)阻断剂格列苯脲(Glibenclamide,Gli)、钙激活钾通道(K_Ca)阻断剂四乙胺(tetraethylammonium,TEA)可部分阻断Res的作用(P<0.05),电压依赖性钾通道(K_V)阻断剂4-氨基吡啶(4-aminopyridine,4-AP)、内向整流钾通道(K_IR)阻断剂氯化钡(barium chloride,BaCl₂)、乙酰胆碱(ACh)调节钾通道(K_ACh)阻断剂阿托品(Atropine)不能阻断Res的作用。结论：Res可呈剂量依赖性减慢HR、减弱CF,其作用是通过开放K_ATP及K_Ca有关,与K_V,K_IR和K_ACh无关。

Abstract:Objective: To study the effects of resvaratrol derivatives on spontaneous HR and CF of isolated guinea pig atrium. Method: The dose-effect curve of resvaratrol was observed. The possible mechanism of potassium channels responsible for changes of CF and HR after administering with resvaratrol was measured. Result: Resvaratrol reduced the spontaneous HR and weakened the CF in a dose-dependent manner ranging from 10^-6 to 3×10^-4mol·L^-1 (P<0.05). As compared with Res group, the effects were partly blocked by Gli (P<0.05) and TEA (P<0.01), but not blocked by 4-AP, BaCl₂, Atropine. Conclusion: Resvaratrol can induce negative chronotropic action and negative inotropic action. The mechanism (s) may relate to the opening of K_ATP and K_Ca.

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