Abstract:Objective: To study the chemical constituents of Pseudostellaria heterophylla. Method: The compounds were isolated by silica gel and Sephadex LH-20 column chromatography and purified by recrystallization. Their structures were elucidated on the basis of physicochemical properties and spectral analysis. Result: Seven compounds were identified as 2-minalin (1), 3-furfuryl pyrrole-2-carboxylate (2), ursolic acid (3), acacetin (4), luteolin (5), acacetin 7-O-β-D-glucopyranosyl (6→1)-α-L-rhamnopyranoside (6). Conclusion: Among them, compounds 3-6 were isolated from the plant for the first time.