Abstract:Objective: To study the chemical constituents of Prunella vulgaris. Method: To separate the constituents of P. vulgaris by using various kinds of chromatography and identify their structures on the basis of spectral analysis. Result: Seven compounds were isolated from the spikes of P. vulgaris. Their structures were established as autantiamide acetate (1), rhein (2), tanshinone I (3), danshensu (4), stigmast-7, 22-dien-3-one (5), 3, 4,/ α-trihydroxy-methyl phenylpropionate (6), butyl rosmarinate (7). Conclusion: Compounds 1-4 were isolated from this genus for the first time.