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期 刊 ：中国中药杂志 2005年 30卷 11期 页码：855.

关键词：葛根总黄酮；尼尔雌醇；鼻黏膜；凋亡；

Keywords:nylestriol, purariae isoflavone, flow cytometry, nasal mucosas, ovaliectomized rats, apoptosis,

摘 要 ：目的:观察卵巢切除诱导大鼠鼻黏膜上皮细胞凋亡,研究尼尔雌醇、葛根总黄酮对此的保护作用。方法:将60只大鼠随机分为4组:正常对照组,卵巢切除组,卵巢切除+尼尔雌醇组,卵巢切除+葛根总黄酮组。各组动物分期分3批处死,流式细胞仪检测各组动物鼻中隔黏膜的早期凋亡细胞。结果:卵巢切除后,大鼠鼻黏膜的早期凋亡细胞增多。葛根总黄酮、尼尔雌醇干预组与正常组比较,早期凋亡细胞无明显改变。结论:葛根总黄酮和尼尔雌醇可通过减少凋亡细胞的百分率而保护鼻黏膜免受雌激素缺乏的损害。

Abstract:Objective: To study the protective effect of purariae isoflavone on apoptosis cells of atrophic nasal mucosas in ovariectomized rats. Method: 60 rats were divided into four groups as control, ovariectomized, ovariectomized + nylestriol (O+ N) and ovariectomized + purariae isoflavone (O + P), each with 15 rats. Earlier apotosis cells of mucosas taken from nasal septum were measured with flow cytometry. Result: Compared with control group, and the number of apoptosis cells of mucosas increased after being ovariectomized, and the number of apoptosis cells of mucosas in O + N and O + D group didn‘ t change. Conclusion: Nylestriol and purariae isoflavone might have effects on protecting cells of mucosas from lacking of estrogen by decreasing apoptosis cells in ovariectomized rats.

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