Abstract:Objective: To study the chemical constituents from the fruits of Paliurus ramosissimus. Method: The constituents of P. ramosissimus were separated with various chromatographic techniques and their structures were elucidated by means of spectral analysis and physico-chemical properties. Result: Nine compounds were isolated and identified as umbelliferone (1), scoparone (2), aurapten (3), bergapten (4), isopimpinellin (5), byakangelicin (6), xanthotoxol (7), isosakuranin (8), poncirin (9). Conclusion: Compounds 1-9 were isolated from the fruits of P. ramosissimus for the first time.