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期 刊 ：中国中药杂志 2006年 31卷 23期 页码：1961.

关键词：大青叶；生物碱；芳香酸；木脂素；黄酮；

Keywords:Isatis indigotica, alkaloids, organic acids, lignans, flavones,

摘 要 ：目的：对十字花科植物菘蓝Isatis indigotica Fort.叶的化学成分进行研究。方法：大青叶的干燥叶用80%乙醇提取,依次用石油醚,醋酸乙酯,正丁醇萃取,对醋酸乙酯部分采用硅胶、Sephadex LH-20, Rp-8, Rp-18柱色谱进行分离纯化,通过波谱数据分析(MS,¹H-NMR,¹³C-NMR)进行结构鉴定。结论：从醋酸乙酯部分分离鉴定了11个化合物,其中5个生物碱,2个芳香酸,2个木脂素,2个黄酮。分别鉴定为：10H-indolo［3,2-b］quinoline(1),靛玉红(2),4(3H)-喹唑酮(3),(E)-3-(3′,5′-dimethoxy-4′-hydroxybenzylidene)-2-indolinone(4),deoxyvascinone(5),苯甲酸(6),邻羟基苯甲酸(7),(-)-落叶松脂素(8),(+)-异落叶松树脂醇(9),异牡荆素(10),6-β-D-glucopyranosyldiosmetin(11)。其中化合物1,4,5,8,9,11为首次从大青叶中分离得到。

Abstract:Objective: To study the chemical constituents of the leaves of Isatis indigotica. Method: The leaves of I. indigotica were extracted with 80% ethanol. The EtOH extract was dispersed in H₂O and extracted with petroleum, EtOAc and BuOH successively. The EtOAc fraction was isolated and purified by column chromatography on silica gel, Sephadex LH-20 and Rp-8, Rp-18. All the compounds were identified on the basis of spectral analyses(including MS, ¹H-NMR, ¹³C-NMR), Result: Eleven compounds were isolated from the leaves of I. indigotica, and structures were characterized as 10H-indolo ［3, 2-b］ quinoline (1), indirubin (2), 4(3H)-quinazo-linone (3), (E)-3-(3′, 5′-dimethoxy-4′-hydroxybenzylidene)-2-indolinone (4), 2, 3-dihydropyrrolo ［2, 1-b］ quinazolin-9(1H)-one (5), benzoic acid (6), o-droxy-benzoic acid (7), (-)-lariciresinol (8), (+)-isolariciresinol (9), isovitexin (10), 6-β-D-glucopyranosyldiosmetin (11). Conclusion: 1, 4, 5, 8, 9, 11 were obtained from the leaves of I. indigotica for the first time.

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