Abstract:Objective: To study the chemical constituents of Acanthus ilicifolius. Method: Chromatographic methods were used to isolate compounds from A. ilicifolius, and chemical and spectroscopic methods were used to elucidate the structures of the isolated compounds. Result: Seven compounds, betaine (1), phenylethyl-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranoside (2), phenylethyl-O-β-D-glucopyranoside (3), acteoside (4), isoacteoside (5), benzyl-O-β-D-glucopyranoside (6) and vanillic acid (7) were obtained. Conclusion: 1, 3, 6 and 7 were obtained from the genus for the first time.