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期 刊 ：中国中药杂志 2005年 30卷 04期 页码：253.

关键词：金钗石斛；内生真菌；多糖含量；总生物碱含量；

Keywords:Dendrobium nobile, endophytic fungi, polysaccharide, alkaloid,

摘 要 ：目的 :研究离体培养时 4种菌根真菌对金钗石斛生长及化学成分含量的影响。方法 :利用纯培养的菌根真菌分别与金钗石斛无菌苗共生培养 ,观测它们对金钗石斛生长及其多糖和总生物碱含量的影响。结果 :与对照相比 ,Mycena sp. (MF2 3 )极显著降低了金钗石斛鲜重的 2 4.9% (P<0 .0 1) ;所有实验菌株对金钗石斛干重均无显著性影响 ;Epulorhiza sp. (MF18)和MF2 3显著提高了金钗石斛的折干率 (P<0.0 5) ,Mycena sp. (MF2 4)极显著提高了金钗石斛的折干率 (P<0. 0 1) ,分别最多提高了 51.3 % ,43 8%和 63. 3 % ;Epulorhizasp (MF15)和MF2 4比对照极显著增加了金钗石斛气生根数量的 4.25倍和 4.13倍 (P<0 .0 1) ;MF2 3极显著减少了金钗石斛基生根数量的46.5% (P<0. 0 1) ;MF15,MF18,MF2 3和MF2 4分别可使金钗石斛多糖含量提高 153 .4% ,52. 1% ,18.5% ,76.7% ;MF2 3使金钗石斛总生物碱含量提高 18.3 %。结论 :在离体培养条件下 ,内生真菌与金钗石斛共生培养能影响金钗石斛的生长和总生物碱的含量 ,提高多糖的含量。

Abstract:Objective: To study the effects of four species of endophytic fungi on the growth and polysaccharide and alkaloid contents of cultured Dendrobium nobile. Method: Each fungal strain was cultured together with D. nobile, and the plant weight as well as the polysaccharide and alkaloid contents were measured. Result: Compared with the control group, Mycena sp.(MF23)was found to decrease the fresh weight of D. nobile by 24.9%(P<0.01). All four species of fungi have no effect on the dry weight of this plant. On the other hand, Epulorhiza sp.(MF18)and MF23 could increase the percent of dry weight of seedlings (P<0.05). Epulorhiza sp.(MF15)and MF24 increased the number of aerial roots by 4.25 times and 4.14 times respectively (P<0.01). MF23 decreased the numbers of basal roots by 46.5%(P<0.01). MF15,MF18,MF23 and MF24 were shown to increase the content of polysacchride by 153.4%,52.1%,18.5% and 76.7%, respectively. MF23 also increased the content of total alkaloid by 18.3%. Conclusion:Endophytic fungi cultured together with D. nobile could affect the growth of this plant; they also increase total alkaloid and polysaccharide contents.

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