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期 刊 ：中国中药杂志 2006年 31卷 24期 页码：2042.

关键词：超临界CO2流体萃取；均匀设计法；高效液相色谱法；隐丹参酮；丹参酮Ⅰ；丹参酮ⅡA；

Keywords:Supercritical-CO fluid extraction, uniform-design, HPLC, cryptotanshinone, tanshinoneⅠ, tanshinoneⅡA,

摘 要 ：目的：探讨超临界CO₂流体萃取法萃取丹参中丹参酮的最佳工艺条件。方法：用高效液相色谱法测定丹参酮含量,以萃取物中3种丹参酮总含量为指标,采用均匀设计法对影响萃取结果的主要因素进行考察。结果：夹带剂用量对萃取结果影响最为显著。最佳萃取工艺参数为：萃取压力30 MPa；萃取温度40 ℃；分离釜Ⅰ压力6 MPa；分离釜Ⅰ温度50 ℃；夹带剂用量10％。结论：超临界CO₂流体萃取法萃取丹参中丹参酮是一种切实可行的方法。所得萃取产物中丹参酮含量高,萃取时间短,操作简便。

Abstract:Objective：To explore the optimal supercritical-CO₂ fluid extraction technique of extracting tanshinones in Radix salviae.Method： The contents of tanshinones were determined by HPLC. Choosing the overall contents of 3 tanshinones as inspecting marker and uniform-design as method, the experimentation was carried out to study the main factors that influence the result of extraction. Result：Among all the 5 inspected factors, the amount of entrainer was most notable. The optimal technique of extraction was： extraction pressure at 30 MPa; extraction temperature at 40 ℃; separation pressure of separating kettleⅠat 6 MPa; separation temperature of separating kettleⅠat 50 ℃; the amount of entrainer at 10%.Conclusion： Adopting supercritical-CO₂ fluid extraction method to extract the tanshinones in Radix salviae was feasible. The contents of tanshinones in the extract were high and the manipulation was simple、convenient and time saving.

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