Abstract:From Podocarpus imbricatus five compounds were isolated and identified as 2″-O-rhamnosylvitexin (Ⅰ), hinokiflavone (Ⅱ), ecdysterone (Ⅲ),-β-sitosteryl heptadecoate (Ⅳ) and daucosterol(Ⅴ) on the basis of spectral studies (UV, IR,HNMR,13CNMR, MS) and chemical reactions. Ⅰ,Ⅱ,Ⅲ,Ⅳ are obtained from this plant for the first time, and Ⅳ is obtained as β-sitosteryl odd number carboxylic ester for the first time.