Abstract:Five compounds were isolated from the fruits of Vitex trifolia and identified on the basis of spectral data and chemical methods to be p-hydroxybenzoic acid .β-sitosterol β-sitosterol-3-O-glucoside. castiein and. 3, 6, 7-trimethylquercetagetin. Except β-sitosterol all of the compounds were obtained from the fruits of the plant for the firsttime.