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期 刊 ：中国中药杂志 1999年 24卷 03期 页码：176.

关键词：虎杖；黄芪；HSV-1F株；HEp-2细胞；中效作用原理；

Keywords:Rhizoma Polygoni Cuspidati, Radix Astragali, HSV 1 F strain, HEp 2 cell, median effect principle,

摘 要 ：目的：为了研制抗病毒新药，观察了虎杖、黄芪联用抗ＨＳＶ-１的药效。方法：用空斑减数实验及中效作用原理对药效进行了分析。结果：在ＨＥｐ-２细胞系统中，以虎杖／黄芪＝１（ＥＤ_５０）／１（ＥＤ_５０）为首选的联用比。①对ＨＳＶ １Ｆ株的直接杀灭，空斑减数率≤８０％时，合用指数＜１．０，有明显的协同作用。②对ＨＳＶ-１Ｆ株增殖的抑制，空斑减数率≤６０％时，合用指数＜１．０，有协同作用。③对ＨＳＶ-１Ｆ株感染的阻断，空斑减数率从２０％～９０％，合用指数＜１．０，协同作用极为显著，是首选的联用比。结论：两药联用治疗指数达10³，细胞毒作用并不相加，对于抗ＨＳＶ-１而言，是有利的药物联用组合。

Abstract:Objective:The clinical action of combined use of Rhizoma Polygoni Cuspidati and Radix Astragali on HSV 1 was investigated with a view to developing a new antiviral drug.Method:The action was analyzed by way of plaque reduction assay and median effect principle.Results:In the HEp 2 cell system, if the combination ratio of Rhizoma Polygoni Cuspidati and Radix Astragali was 1(ED ₅₀ )∶1(ED ₅₀ ) then, (1)In directly annihilating HSV 1 F strain, when the plaque reduction rate was 20%～80%,and the combination index was<1.0, there was synergism.(2) In inhibiting the multiplication of HSV 1 F strain, when the plaque reduction rate was 20%～60%, and the combination index was<1.0, there was also synergism. (3)In blockading HSV 1 F strain infection, when the plaque reduction rate was 20%～90% and the combination index was<1.0, there was synergism.So this ratio of 1(ED ₅₀ )∶1(ED ₅₀ ) should be the first choice for combination.Conclusion: The treatment index of the above two Chinese medicinal herbs equals 10 3, and the cytotoxicity is not potentiated,indicating that the combination is helpful as a virucide for HSV 1 F strain.

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