Abstract:Objective To investigate the chemical constituents of Myrica nana collected in Yunnan Province, China.Methods Column chromatography techniques were used for separation and purification of the compounds and extensive spectral analyses including 2D NMR spectrum were employed for structural elucidation.Results Eleven compounds were isolated from the roots of M nana.Structures of these compounds were identified by 13C-NMR,1H-NMR,and MS as following:2α,3β-dihydroxyl-lup-20(29)-en-28(19)-lactone(Ⅰ),2α,3β-isopropylidene-lup-20(29)-en-28(19)-lactone(Ⅱ),longispinogenin(Ⅲ),masilinic acid(Ⅳ),oleanolic acid(Ⅴ),myricadiol(Ⅵ),arjunolic acid(Ⅶ)、arjunglucoside II(Ⅷ),dihydroxyligustilide(Ⅸ),hydropiperoside(Ⅹ),6‘-O-acylclionastero-3-O-glucoside(Ⅺ).Conclusion Compound Ⅰ is a new compound named lupeolenolide; Compound II is a new artifact of I produced during isolation procedure,named isopropyl-lupeolenolide and compounds Ⅲ,Ⅷ-Ⅺ are isolated from the roots of M.nana for the first time.