全 文 ：RP-HPLC 法测定中药铁苋菜中没食子酸的含量
王建春　房克慧■　潘金火■■　罗　兰■■■　(江苏省张家港市中医院 ,张家港　215600
■　江苏省扬州市药品检验所 , 扬州　225000　■■南京中医药大学 , 南京　210029　■■■陕西省宝鸡市药检所 , 宝鸡　721001)
摘　要　目的:测定中药铁苋菜中没食子酸的含量。方法:运用 RP-HPLC 测定法。结果:铁苋菜中没食子酸的
平均含量为0.543%, RSD为 2.78%。结论:铁苋菜中含有活性成分没食子酸 ,本实验可为定量地控制该药材的质量
提供参考。
　　关键词　铁苋菜　没食子酸　RP-HPLC法　含量测定
　　中图号　R284.1
　　中药铁苋菜为大戟科植物铁苋菜(Acalypha
austral is L.)的全草 ,性凉味苦 ,具有清热利湿 、杀虫
止血之效 ,主治痢疾 、腹泻及各种出血证 ,其活性成
分之一为没食子酸 ,我们用反相 HPLC 法测定了药
材中没食子酸的含量 ,方法可靠 、灵敏 ,重现性好。
1　仪器与试药
1.1　仪器
高效液相色谱仪 Waters Model 510 泵;SIL-1A
六通进样阀;Waters 490型紫外检测器;SP4270 型
微处理机 。
1.2　试药
铁苋菜饮片由江苏省中医院提供(经中药鉴定
室刘训红副教授鉴定)。没食子酸对照品由南京市
植物研究所提供 ,纯度为 98.6%。乙腈为色谱纯
(上海振兴化工厂);其它试剂均为 AR级 。
2　方法与结果
2.1　色谱条件
YWG-G18柱(150 mm×4.6 mm ,5μm)(大连化
学物理研究所);流动相:乙腈∶0.032 mol/L 四丁基
硫酸氢氨∶水(5∶15∶80),流速:0.5 ml/min;检测波
长:275 nm[ 1] ;灵敏度:0.05 AUF;进样量:20 μl;理
论塔板数按没食子酸峰计算应不低于 4000 ,在此条
件下指标成分分离良好。
2.2　标准曲线的绘制
精密称取干燥至恒重的没食子酸对照品约 20
mg 置10 ml量瓶中 ,用50%甲醇溶解并稀释至刻度
(储备液),精吸储备液 20 、40 、60 、80 、100 、120 μl分
别于 1 ml量瓶中 ,用 50%甲醇定容 ,各进样 20 μl ,
以峰面积积分值 A对进样量 C(μg)作图得回归方
程:
A=431815 C -5386.4(r=0.9999),进样量在
0.186 ～ 4.896μg 间线性关系良好。
2.3　样品测定
先用药典法测定铁苋菜饮片的含水量 ,然后精
密称取药材粉末约 1.5 g(按比例减去含水量),再用
石油醚超声脱脂 3次 ,残渣蒸干后 ,用 50%甲醇 15
ml超声提取 3次 ,每次 30 min ,滤液定容至 50 ml ,
进样 20μl。由回归方程计算样品中没食子酸的含
量 ,结果如下 。
表 1　铁苋菜中没食子酸的含量(n =5)
样品干重(g) 含量(%) 平均含量(%) RSD(%)
1.0327 0.550
1.1245 0.522
1.1863 0.540 0.543 2.78
1.0677 0.539
0.9862 0.563
2.4　加样回收率试验
精密称取药材粉末约 1.5 g 共 5 份(按比例减
去含水量),各精密加入标准储备液 3 ml ,然后按样
品测定项下的方法测定。结果表明 ,平均加样回收
率为 95.04%, RSD为 2.33%。
2.5　稳定性试验
取在室温下放置的标准液分别进样 5 次(每隔
6 h 1次),发现5次峰面积积分值基本不变 , RSD为
3.47%,由此认为指标成分在 24 h内性质稳定。
3　讨论
没食子酸是共认的抗菌 、收敛 、止泻活性成分 ,
本文用先进的测试手段测定了铁苋菜中没食子酸的
含量 ,为定量地控制该药材的质量提供了科学的方
法和依据。
参考文献
1　章促懿 ,金裕田 , 边崇安.反相 HPLC 法测定溃结灌
肠液中没食子酸和绿原酸的含量.中国生化药物杂志 , 1993 ,
(4)∶62
(收稿日期:1997-12-21)
—224— 　　南京中医药大学学报 1998 年 7 月第 14 卷第 4 期　JOURNAL OF NANJING UNIVERSIT Y OF TCM Vol.14 No.4 July.1998
DOI :10.14148/j.issn.1672-0482.1998.04.014
Fluid for Reducing Stomach Fire significangly reduced the levels of plasma TXB2 and 6-Keto-PGF 1αand thei r ra-
tio as w ell.Conclusion:The therapeutic ef fect of chronic gast ritis by Rhizoma Pinel liae Fluid fo r Reducing
S tomach Fire may result f rom the promotion of blood flow in gastric mucosa and from the increase of the supply
of oxygen and energy to gast ric mucosa.
　　KEYWORDS:Rizoma Pinel liae Fluid for Reducing S tomach Fire , chronic gast ritis , TXB2 ,6-Keto-PGF 1α
(Page 220)
RP-HPLC in Determining the Contents of Gall ic Acid in Tie Xian Cai
Wang Jianchun , Fang Kehui ■ , Pan Jinhuo■■ , Luo Lan■■■
　(Zhangjiagang Hospital of Traditional Chinese Medicine , Zhangjiagang , Jiangsu , 215600 , ■Yangzhou Institute of
Drug Control , Yangzhou ,Jiangsu ,225000 , ■■Nanjing University of Traditional Chinese Medicine , Nanjing ,210029 ,
■■■Baoji Instituteof Drug Control ,Baoji , Shaanxi ,721001)　　Abstract　Objective:to determine the contents of gallic acid in Tie Xian Cai(Herba Acalyphae).Methods:RP-HPLC
was conducted.Results:The mean contents of gallic acid in Tie Xian Cai was 0.543%.RSD was 2.78%.Conclusion:Tie Xi-
an Cai contains the active element gallic acid.The results of this test can be used in the quality contrl of Tie Xian Cai in
quantity.　　KEYWORDS:Tie Xian Cai , gallic acid ,RP-HPLC , content determination
(Page 224)
Extraction Process of Ge Gen
Liu Chanming , Yang Hongyuan , Qin Chunyan■ , Yu Shanmei■
(Research Institute of Traditional Chinese Medicine ,Changzhou Hospital of Traditional Chinese Medicine ,
Changzhou , Jiangsu ,213003 ,■Intern of Grade96 ,Nanjing University of Traditional Chinese Medicine ,Nanjing ,210029)
　　Abstract 　Objective;To study the ex traction proess of Ge Gen(Radix Puerariae)to determine the ideal
ex traction condition.Methods:Orthogonal test was conducted to study the ext raction process of total flavone of
Ge Gen.With the content of the to tal flavone as the index , f ive factors were taken into consideration:size of the
herbal medicine , solvent concentration , quant ity , ex traction duration , and number of ex traction times.Results:
The ideal ext ract ion condit ions wer A1 、B1 、C1 、D3 , and E3 , that is , 10 times of 70%alchol w as added to the her-
hal pieces , and ref lux ex traction w as conducted 7 times.0.5 h.each time.Conclusion:The establishment of the
ideal ex traction condition of Ge Gen has laid a scient if ic foundaton fo r mass product ion.
　　KEYWORDS:Ge Gen , o rthogonal tex t design , total f lavone of Ge Gen , ext ract ion process (Page 225)
Preparation and Content Control of Syrup for Nourishing Yin and Clearing the Lung-heat
Yu Luojun , Huang Lianzhong■ , Liu Hanqing■■
(Jintan People s Hospi tal , J intan , J iangsu ,213200 , ■Jiangyan Medicinal Corporation ,
J iangyan , Jiangsu , 225500 , ■■Nanj ing University of Tradit ional Chinese Medicine , Nanjing , 210029)
　　Abstract　Objective:To make the medicine easier to take and to stabilize the quality .Methods:The herbs
w ere ext racted respectively and decocted together , and the sy rup ex tract ,Sy rup Ex tract fo r Nourishing Yin and
Clearing the Lung-heat(Yang Yin Qing Fei Gao)was made instead of the sof t ext ract.Ultraviolet spect ropho-
tometry w as conducted to de-termine the contents of paeonol.Results:The Changed dosage form met the require-
ment stipulated by the general rule for the preparation of syrup in the Pharmacopeia.The content of paeonol was
(0.06±0.01)%.Conclusion:The quality of the sy rup is stable , and its therapeut ic effects are satisfacto ry.　　KEYWORDS:Sy rup for Nourishing Yin and Clearing the Lung heat(Yang Yin Qing Fei Gao), prepara-
tion , content determination (Page 227)
Treatment of Opiumism in WANG S MEDICAL RECORD
Xu Li
(Doctoral Student of Grade 96 , Nanjing Universi ty of Tradit ional Chinese Medicine , Nanj ing ,210029)　 　Abstract　In the book WANG S MEDICAL RECORD(WANG SHI YI CUN),brilliant opinions are ex-
pressed on the pathogenesis , diagnosis , t reatment , prognosis , and contraindication of opiumism.The experience
discussed in the book is of great help to the TCM treatment of opiumism.We should learn the experience and
achievements f rom the ancient medical practit ioners so as to do the research on opiumism well　　KEYWORDS:WANG S MEDICAL RECORD , opiumism (Page 234)
Translated by Shi Yunzhong
南京中医药大学学报 1998 年第 14 卷第 4 期