Protoplasts Isolation Conditions of Melilotoides ruthenica Callus Dissociation and Culture of Callus Protoplasts of Medicago sativa L. Enzymolysis and Isolation Conditions of Protoplasts from Cotyledons and Calli in Lotus corniculatus Isolation and Cultivation of Protoplasts of Liriodendron tulipifera Effects of Different Enzymolysis Conditions on the Cotyledons Protoplast Isolation of Alfalfa